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Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus)
Fan, Zhaofei1,2,3; Zou, Yuxia1,2; Liang, Dongdong1,2,3; Tan, Xungang1,2; Jiao, Shuang1,2; Wu, Zhihao1,2; Li, Jun1,2; Zhang, Peijun1,2; You, Feng1,2
2019
Source PublicationREPRODUCTION FERTILITY AND DEVELOPMENT
ISSN1031-3613
Volume31Issue:11Pages:1742-1752
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractAs an important maricultured fish, the olive flounder Paralichthys olivaceus shows sex-dimorphic growth. Thus, the molecular mechanisms involved in sex control in P. olivaceus have attracted researchers' attention. Among the sex-related genes, forkhead box protein L2 (foxl2) exhibits significant sex-dimorphic expression patterns and plays an important role in fish gonad differentiation and development. The present study first investigated the expression levels and promoter methylation dynamics of foxl2 during flounder gonad differentiation under treatments of high temperature and exogenous 17 beta -oestradiol (E2). During high temperature treatment, the expression of flounder foxl2 may be repressed via maintenance of DNA methylation. Then, flounder with differentiated testis at Stages I-II were treated with exogenous 5ppm E2 or 5ppm E2+150ppm trilostane (TR) to investigate whether exogenous sex hormones could induce flounder sex reversal. The differentiated testis exhibited phenotypic variations of gonadal dysgenesis with upregulation of female-related genes (foxl2 and cytochrome P450 family 19 subfamily A (cyp19a)) and downregulation of male-related genes (cytochrome P450 family 11 subfamily B member 2 (cyp11b2), doublesex- and mab-3 related transcription factor 1 (dmrt1), anti-Mullerian hormone (amh) and SRY-box transcription factor 9 (sox9)). Furthermore, a cotransfection assay of the cells of the flounder Sertoli cell line indicated that Foxl2 was able alone or with nuclear receptor subfamily 5 group A member 2 (Nr5a2) jointly to upregulate expression of cyp19a. Moreover, Foxl2 and Nr5a2 repressed the expression of dmrt1. In summary, Foxl2 may play an important role in ovarian differentiation by maintaining cyp19a expression and antagonising the expression of dmrt1. However, upregulation of foxl2 is not sufficient to induce the sex reversal of differentiated testis.
KeywordDNA methylation high temperature and exogenous sex hormones gene expression gonad development
DOI10.1071/RD18233
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[41276171] ; National Key R & D Program of China[2018YFD0900202] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaDevelopmental Biology ; Reproductive Biology ; Zoology
WOS SubjectDevelopmental Biology ; Reproductive Biology ; Zoology
WOS IDWOS:000489057300010
PublisherCSIRO PUBLISHING
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163384
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fan, Zhaofei,Zou, Yuxia,Liang, Dongdong,et al. Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus)[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2019,31(11):1742-1752.
APA Fan, Zhaofei.,Zou, Yuxia.,Liang, Dongdong.,Tan, Xungang.,Jiao, Shuang.,...&You, Feng.(2019).Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus).REPRODUCTION FERTILITY AND DEVELOPMENT,31(11),1742-1752.
MLA Fan, Zhaofei,et al."Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus)".REPRODUCTION FERTILITY AND DEVELOPMENT 31.11(2019):1742-1752.
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