Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus) | |
Fan, Zhaofei1,2,3 ; Zou, Yuxia1,2; Liang, Dongdong1,2,3; Tan, Xungang1,2; Jiao, Shuang1,2; Wu, Zhihao1,2; Li, Jun1,2; Zhang, Peijun1,2; You, Feng1,2
| |
| 2019 | |
| 发表期刊 | REPRODUCTION FERTILITY AND DEVELOPMENT
 |
| ISSN | 1031-3613 |
| 卷号 | 31期号:11页码:1742-1752 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 摘要 | As an important maricultured fish, the olive flounder Paralichthys olivaceus shows sex-dimorphic growth. Thus, the molecular mechanisms involved in sex control in P. olivaceus have attracted researchers' attention. Among the sex-related genes, forkhead box protein L2 (foxl2) exhibits significant sex-dimorphic expression patterns and plays an important role in fish gonad differentiation and development. The present study first investigated the expression levels and promoter methylation dynamics of foxl2 during flounder gonad differentiation under treatments of high temperature and exogenous 17 beta -oestradiol (E2). During high temperature treatment, the expression of flounder foxl2 may be repressed via maintenance of DNA methylation. Then, flounder with differentiated testis at Stages I-II were treated with exogenous 5ppm E2 or 5ppm E2+150ppm trilostane (TR) to investigate whether exogenous sex hormones could induce flounder sex reversal. The differentiated testis exhibited phenotypic variations of gonadal dysgenesis with upregulation of female-related genes (foxl2 and cytochrome P450 family 19 subfamily A (cyp19a)) and downregulation of male-related genes (cytochrome P450 family 11 subfamily B member 2 (cyp11b2), doublesex- and mab-3 related transcription factor 1 (dmrt1), anti-Mullerian hormone (amh) and SRY-box transcription factor 9 (sox9)). Furthermore, a cotransfection assay of the cells of the flounder Sertoli cell line indicated that Foxl2 was able alone or with nuclear receptor subfamily 5 group A member 2 (Nr5a2) jointly to upregulate expression of cyp19a. Moreover, Foxl2 and Nr5a2 repressed the expression of dmrt1. In summary, Foxl2 may play an important role in ovarian differentiation by maintaining cyp19a expression and antagonising the expression of dmrt1. However, upregulation of foxl2 is not sufficient to induce the sex reversal of differentiated testis. |
| 关键词 | DNA methylation high temperature and exogenous sex hormones gene expression gonad development |
| DOI | 10.1071/RD18233 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[41276171] ; National Key R & D Program of China[2018YFD0900202] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[41276171] ; National Key R & D Program of China[2018YFD0900202] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] |
| WOS研究方向 | Developmental Biology ; Reproductive Biology ; Zoology |
| WOS类目 | Developmental Biology ; Reproductive Biology ; Zoology |
| WOS记录号 | WOS:000489057300010 |
| 出版者 | CSIRO PUBLISHING |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/163384 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Fan, Zhaofei,Zou, Yuxia,Liang, Dongdong,et al. Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus)[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2019,31(11):1742-1752. |
| APA | Fan, Zhaofei.,Zou, Yuxia.,Liang, Dongdong.,Tan, Xungang.,Jiao, Shuang.,...&You, Feng.(2019).Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus).REPRODUCTION FERTILITY AND DEVELOPMENT,31(11),1742-1752. |
| MLA | Fan, Zhaofei,et al."Roles of forkhead box protein L2 (foxl2) during gonad differentiation and maintenance in a fish, the olive flounder (Paralichthys olivaceus)".REPRODUCTION FERTILITY AND DEVELOPMENT 31.11(2019):1742-1752. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Roles of forkhead bo（1065KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论