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Enterococcus faecalis alpha 1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification
Li, Yanhong1; Li, Riyao1; Yu, Hai1; Sheng, Xue1; Wang, Jing1,2; Fisher, Andrew J.1,3; Chen, Xi1
2019-10-08
Source PublicationFEBS LETTERS
ISSN0014-5793
Pages13
Corresponding AuthorChen, Xi(xiichen@ucdavis.edu)
AbstractWhile multiple alpha 1-2-mannosidases are necessary for glycoprotein N-glycan maturation in vertebrates, a single bacterial alpha 1-2-mannosidase can be sufficient to cleave all alpha 1-2-linked mannose residues in host glycoprotein N-glycans. We report here the characterization and crystal structure of a new alpha 1-2-mannosidase (EfMan-I) from Enterococcus faecalis, a Gram-positive opportunistic human pathogen. EfMan-I catalyzes the cleavage of alpha 1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. Its 2.15 angstrom resolution crystal structure reveals a two-domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan-I as an effective catalyst for in vitro N-glycan modification of glycoproteins with high-mannose-type N-glycans.
Keywordalpha-mannosidase crystal structure glycoprotein modification mannosidase N-glycan enzymatic modification
DOI10.1002/1873-3468.13618
Indexed BySCI
Language英语
Funding ProjectUnited State Defense Threat Reduction Agency[HDTRA1-15-1-0054] ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences[DE-AC02-76SF00515] ; DOE Office of Biological and Environmental Research ; National Institutes of Health, National Institute of General Medical Sciences[P41GM103393]
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS IDWOS:000489181900001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163349
Collection实验海洋生物学重点实验室
Corresponding AuthorChen, Xi
Affiliation1.Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
3.Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA
Recommended Citation
GB/T 7714
Li, Yanhong,Li, Riyao,Yu, Hai,et al. Enterococcus faecalis alpha 1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification[J]. FEBS LETTERS,2019:13.
APA Li, Yanhong.,Li, Riyao.,Yu, Hai.,Sheng, Xue.,Wang, Jing.,...&Chen, Xi.(2019).Enterococcus faecalis alpha 1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification.FEBS LETTERS,13.
MLA Li, Yanhong,et al."Enterococcus faecalis alpha 1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification".FEBS LETTERS (2019):13.
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