IOCAS-IR  > 海洋生态与环境科学重点实验室
An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria
Chen, Hao1,3,4; Wang, Minxiao1,2,3,4; Zhang, Huan1,3,4; Wang, Hao1,3,4; Lv, Zhao5; Zhou, Li1,3,4; Zhong, Zhaoshan1,5; Lian, Chao1,3,4; Cao, Lei1,3,4; Li, Chaolun1,2,3,4,5
2019-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume93Pages:354-360
Corresponding AuthorLi, Chaolun(lcl@qdio.ac.cn)
AbstractAs domain species in seep and vent ecosystem, Bathymodioline mussels has been regarded as a model organism in investigating deep sea chemosymbiosis. However, mechanisms underlying their symbiosis with chemosynthetic bacteria, especially how the host recognizes symbionts, have remained largely unsolved. In the present study, a modified pull-down assay was conducted using enriched symbiotic methane-oxidation bacteria as bait and gill proteins of Bathymodiolus platifrons as a target to isolate pattern recognition receptors involved in the immune recognition of symbionts. As a result, a total of 47 proteins including BpLRR-1 were identified from the pull-down assay. It was found that complete cDNA sequence of BpLRR-1 contained an open reading frame of 1479 bp and could encode a protein of 492 amino acid residues with no signal peptide or transmembrane region but eight LRR motif and two EFh motif. The binding patterns of BpLRR-1 against microbial associated molecular patterns were subsequently investigated by surface plasmon resonance analysis and LPS pull-down assay. Consequently, BpLRR-1 was found with high binding affinity with LPS and suggested as a key molecule in recognizing symbionts. Besides, transcripts of BpLRR-1 were found decreased significantly during symbiont depletion assay yet increased rigorously during symbionts or nonsymbiotic Vibrio alginolyticus challenge, further demonstrating its participation in the chemosynthetic symbiosis. Collectively, these results suggest that BpLRR-1 could serve as an intracellular recognition receptor for the endosymbionts, providing new hints for understanding the immune recognition in symbiosis of B. platifrons.
KeywordBathymodioline mussels Chemosymbiosis Pattern recognition receptors LRR protein Pull-down
DOI10.1016/j.fsi.2019.07.032
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; National Key Research and Development Program of China[2018YFC0310802] ; National Key Research and Development Program of China[2018YFC0310604] ; China Postdoctoral Science Foundation[2017LH023] ; Qingdao Postdoctoral Science Foundation
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000488998300041
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163306
Collection海洋生态与环境科学重点实验室
Corresponding AuthorLi, Chaolun
Affiliation1.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
5.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Hao,Wang, Minxiao,Zhang, Huan,et al. An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:354-360.
APA Chen, Hao.,Wang, Minxiao.,Zhang, Huan.,Wang, Hao.,Lv, Zhao.,...&Li, Chaolun.(2019).An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria.FISH & SHELLFISH IMMUNOLOGY,93,354-360.
MLA Chen, Hao,et al."An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria".FISH & SHELLFISH IMMUNOLOGY 93(2019):354-360.
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