IOCAS-IR  > 实验海洋生物学重点实验室
The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge
Wang, Yilong1,2,3; Wang, Baojie1,2; Shao, Xuqing6; Shao, Jianchun1,2,3; Liu, Mei1,2; Wang, Mengqiang1,2,4; Wang, Lei1,2,5
2019-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume93Pages:517-530
Corresponding AuthorWang, Mengqiang(wangmengqiang@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
AbstractRearing density and disease management are considered as pivotal factors determining shrimp farm productivity and profitability. To systematically investigate the potential mechanisms for density-related differences between disease susceptibility and rearing densities, we conducted comparative transcriptome analysis of the molecular differences between hepatopancreas and intestine of Litopenaeus vannamei under two different rearing densities (800- and 400- shrimp/m(3)) for 15 d and further analyzed the differences in immune response to Vibrio parahaemolyticus E1 (VPE1) raised under two density conditions. Totally 45 different expression genes (DEGs) were identified in the hepatopancreas under two different rearing densities, the DEGs were grouped into four processes or pathways related to animal immune system. Then, exposure to the VPE1 resulted in 639 DEGs, involved into fourteen immune related processes or pathways. In the intestine, seventeen processes or pathways related to the immune system were identified among the 5470 DEGs under two different rearing densities. 279 DEGs were identified post VPE1 challenge, classified into five processes or pathways associated with the immune system. Meanwhile, the results of growth performance, histopathology and the activities of antioxidant enzymes in the hepatopancreas and intestines of shrimp showed that high density decreased weight gain rate (63.20 +/- 1.67% and 18.73 +/- 3.35% in the high and low rearing density groups, respectively), severely destroyed the histopathology and inhibited the antioxidant enzymes activities. This study demonstrated that rearing density in L. vannamei significantly impacts susceptibility to the VPE1, via altered transcriptional challenge responses, and thus higher mortality due to disease.
KeywordRearing density Vibrio parahaemolyticus E1 Transcriptome Histopathology Antioxidant status
DOI10.1016/j.fsi.2019.08.004
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Science and Technology Development Fund Project of Shinan district, Qingdao city, China[2018-4-001-ZH]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000488998300058
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163236
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Mengqiang; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
6.Shandong Cigna Detect Technol Co Ltd, Qingdao 266237, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Yilong,Wang, Baojie,Shao, Xuqing,et al. The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:517-530.
APA Wang, Yilong.,Wang, Baojie.,Shao, Xuqing.,Shao, Jianchun.,Liu, Mei.,...&Wang, Lei.(2019).The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge.FISH & SHELLFISH IMMUNOLOGY,93,517-530.
MLA Wang, Yilong,et al."The effect of rearing density on immune responses of hepatopancreas and intestine in Litopenaeus vananmei against Vibrio paraheamolyticus E1 challenge".FISH & SHELLFISH IMMUNOLOGY 93(2019):517-530.
Files in This Item:
File Name/Size DocType Version Access License
Wang-2019-The-effect(3312KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Yilong]'s Articles
[Wang, Baojie]'s Articles
[Shao, Xuqing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Yilong]'s Articles
[Wang, Baojie]'s Articles
[Shao, Xuqing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Yilong]'s Articles
[Wang, Baojie]'s Articles
[Shao, Xuqing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Wang-2019-The-effect-of-rearing-density-on-im.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.