Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

doi:10.1007/s13131-019-1429-z

IOCAS-IR

	Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina
	Yan, Wenjie 1; Liu, Jiao 2; Seng, Samphal 3; Zhou, Bin 1; Ding, Kuke 4,5
	2019-10-01
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	38 期号:10 页码:111-115
通讯作者	Ding, Kuke(shouding@ccmu.edu.cn)
摘要	The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
关键词	Zostera marina antioxidant enzyme glutathione S-transferase (GST) temperature enzyme activity
DOI	10.1007/s13131-019-1429-z
收录类别	SCI
语种	英语
资助项目	Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration[201704] ; Shandong Provincial Natural Science Foundation of China[ZR2018MD020] ; Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration[201704] ; Shandong Provincial Natural Science Foundation of China[ZR2018MD020]
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000490598500013
出版者	SPRINGER
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163227
专题	中国科学院海洋研究所
通讯作者	Ding, Kuke
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Dept Marine Ecol, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 3.Royal Univ Agr, Fac Fisheries & Aquaculture, Phnom Penh 12101, Cambodia 4.Chinese Ctr Dis Control & Prevent, Natl Inst Radiol Protect, Beijing 100088, Peoples R China 5.Chinese Ctr Dis Control & Prevent, Key Lab Radiol Protect & Nucl Emergency, Beijing 100088, Peoples R China
推荐引用方式 GB/T 7714	Yan, Wenjie,Liu, Jiao,Seng, Samphal,et al. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. ACTA OCEANOLOGICA SINICA,2019,38(10):111-115.
APA	Yan, Wenjie,Liu, Jiao,Seng, Samphal,Zhou, Bin,&Ding, Kuke.(2019).Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina.ACTA OCEANOLOGICA SINICA,38(10),111-115.
MLA	Yan, Wenjie,et al."Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina".ACTA OCEANOLOGICA SINICA 38.10(2019):111-115.

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Yan-2019-Cloning-cha（733KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览