IOCAS-IR
Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina
Yan, Wenjie1; Liu, Jiao2; Seng, Samphal3; Zhou, Bin1; Ding, Kuke4,5
2019-10-01
Source PublicationACTA OCEANOLOGICA SINICA
ISSN0253-505X
Volume38Issue:10Pages:111-115
Corresponding AuthorDing, Kuke(shouding@ccmu.edu.cn)
AbstractThe response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
KeywordZostera marina antioxidant enzyme glutathione S-transferase (GST) temperature enzyme activity
DOI10.1007/s13131-019-1429-z
Indexed BySCI
Language英语
Funding ProjectOpen Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration[201704] ; Shandong Provincial Natural Science Foundation of China[ZR2018MD020]
WOS Research AreaOceanography
WOS SubjectOceanography
WOS IDWOS:000490598500013
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163227
Collection中国科学院海洋研究所
Corresponding AuthorDing, Kuke
Affiliation1.Ocean Univ China, Coll Marine Life Sci, Dept Marine Ecol, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
3.Royal Univ Agr, Fac Fisheries & Aquaculture, Phnom Penh 12101, Cambodia
4.Chinese Ctr Dis Control & Prevent, Natl Inst Radiol Protect, Beijing 100088, Peoples R China
5.Chinese Ctr Dis Control & Prevent, Key Lab Radiol Protect & Nucl Emergency, Beijing 100088, Peoples R China
Recommended Citation
GB/T 7714
Yan, Wenjie,Liu, Jiao,Seng, Samphal,et al. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J]. ACTA OCEANOLOGICA SINICA,2019,38(10):111-115.
APA Yan, Wenjie,Liu, Jiao,Seng, Samphal,Zhou, Bin,&Ding, Kuke.(2019).Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina.ACTA OCEANOLOGICA SINICA,38(10),111-115.
MLA Yan, Wenjie,et al."Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina".ACTA OCEANOLOGICA SINICA 38.10(2019):111-115.
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