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Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure
Ren, Zhonghua1,3; Liu, Jinhu1,2; Huang, Wei4; Cao, Liang1,2; Cui, Wenting1,3; Dou, Shuozeng1,2,3
2019-11-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
Volume225Pages:11
Corresponding AuthorCao, Liang(caoliang@qdio.ac.cn) ; Dou, Shuozeng(szdou@qdio.ac.cn)
AbstractMethylmercury (MeHg) is a highly toxic contaminant in coastal environments and poses threats to marine fish in early life stages (ELSs). However, MeHg toxicity to fish embryos and larvae is not well investigated. This study investigated the antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae exposed to waterborne MeHg (0, 0.1, 1.0 and 10.0 mu g L-1) for 35 days, from embryogenesis to settlement. The results revealed that metal accumulation in the larvae was positively correlated with MeHg concentration, reduced larval growth and survival. The activities of catalase and glutathione reductase were significantly increased at 10.0 mu g L-1, while glutathione peroxidase activity and lipid peroxidation level were significantly increased at concentrations over 1.0 mu g L-1. The corresponding antioxidant-related genes were upregulated under MeHg exposure (cat and gpx at 10.0 mu g L-1; gr over 1.0 mu g L-1). Lysozyme content was significantly increased, but immunoglobulin M content was significantly decreased at 10.0 mu g L-1. The immune-related genes were significantly upregulated (hsp70 at 0.1 and 10.0 mu g L-1; lzm and il-1 beta over 1.0 mu g L-1; tnf-alpha and il-6 at 10.0 mu g L-1) or downregulated (igm, over 0.1 mu g L-1). Overall, MeHg exposure induced oxidative stress and caused immunotoxicity at concentrations over 1.0 mu g L-1 and 10.0 mu g L-1, respectively. The transcription of selected genes correlated with the corresponding biochemical markers in response to MeHg toxicity. These findings improve our knowledge to better understand the mechanisms by which marine fish at ELSs cope with oxidative stress and immunotoxicity induced by MeHg.
KeywordMetal accumulation Larval growth Biochemical markers Gene transcription Oxidative stress Immunotoxicity
DOI10.1016/j.cbpc.2019.108589
Indexed BySCI
Language英语
Funding ProjectNational Key Basic Research Program[2015CB453302] ; National Natural Science Foundation of China[41406176] ; National Natural Science Foundation of China[41306112]
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS IDWOS:000488304600018
PublisherELSEVIER SCIENCE INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163221
Collection海洋生态与环境科学重点实验室
Corresponding AuthorCao, Liang; Dou, Shuozeng
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.State Ocean Adm, Inst Oceanog 2, Key Lab Marine Ecosyst & Biogeochem, Hangzhou 310000, Zhejiang, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ren, Zhonghua,Liu, Jinhu,Huang, Wei,et al. Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2019,225:11.
APA Ren, Zhonghua,Liu, Jinhu,Huang, Wei,Cao, Liang,Cui, Wenting,&Dou, Shuozeng.(2019).Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,225,11.
MLA Ren, Zhonghua,et al."Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 225(2019):11.
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