Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Transcriptome and Gene Coexpression Network Analyses of Two Wild Populations Provides Insight into the High-Salinity Adaptation Mechanisms of Crassostrea ariakensis | |
Liu, Xingyu1,3; Li, Li1,2,5,6; Li, Ao1,3; Li, Yingxiang1,4,5,6; Wang, Wei1,2,4,5,6; Zhang, Guofan1,4,5,6 | |
2019-10-01 | |
发表期刊 | MARINE BIOTECHNOLOGY |
ISSN | 1436-2228 |
卷号 | 21期号:5页码:596-612 |
通讯作者 | Li, Li(lili@qdio.ac.cn) |
摘要 | Crassostrea ariakensis naturally distributes in the intertidal and estuary region with relative low salinity ranging from 10 to 25 parts per thousand. To understand the adaptive capacity of oysters to salinity stress, we conducted transcriptome analysis to investigate the metabolic pathways of salinity stress effectors in oysters from two different geographical sites, namely at salinities of 16, 23, and 30 parts per thousand. We completed transcriptome sequencing of 18 samples and a total of 52,392 unigenes were obtained after assembly. Differentially expressed gene (DEG) analysis and weighted gene correlation network analysis (WGCNA) were performed using RNA-Seq transcriptomic data from eye-spot larvae at different salinities and from different populations. The results showed that at moderately high salinities (23 and 30 parts per thousand), genes related to osmotic agents, oxidation-reduction processes, and related regulatory networks of complex transcriptional regulation and signal transduction pathways dominated to counteract the salinity stress. Moreover, there were adaptive differences in salinity response mechanisms, especially at high salinity, in oyster larvae from different populations. These results provide a framework for understanding the interactions of multiple pathways at the system level and for elucidating the complex cellular processes involved in responding to osmotic stress and maintaining growth. Furthermore, the results facilitate further research into the biological processes underlying physiological adaptations to hypertonic stress in marine invertebrates and provide a molecular basis for our subsequent search for high salinity-tolerant populations. |
关键词 | High-salinity adaptation Wild populations Larvae transcriptome Weighted gene correlation network analysis Crassostrea ariakensis |
DOI | 10.1007/s10126-019-09896-9 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050402] ; National Key R&D Program of China[2018YFD0900304] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050402] ; National Key R&D Program of China[2018YFD0900304] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] |
WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS记录号 | WOS:000487127600002 |
出版者 | SPRINGER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/162996 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Li, Li |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Key Lab Ecol Mariculture, Qingdao, Shandong, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Liu, Xingyu,Li, Li,Li, Ao,et al. Transcriptome and Gene Coexpression Network Analyses of Two Wild Populations Provides Insight into the High-Salinity Adaptation Mechanisms of Crassostrea ariakensis[J]. MARINE BIOTECHNOLOGY,2019,21(5):596-612. |
APA | Liu, Xingyu,Li, Li,Li, Ao,Li, Yingxiang,Wang, Wei,&Zhang, Guofan.(2019).Transcriptome and Gene Coexpression Network Analyses of Two Wild Populations Provides Insight into the High-Salinity Adaptation Mechanisms of Crassostrea ariakensis.MARINE BIOTECHNOLOGY,21(5),596-612. |
MLA | Liu, Xingyu,et al."Transcriptome and Gene Coexpression Network Analyses of Two Wild Populations Provides Insight into the High-Salinity Adaptation Mechanisms of Crassostrea ariakensis".MARINE BIOTECHNOLOGY 21.5(2019):596-612. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Liu-2019-Transcripto(3289KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
Transcriptome and Ge(3295KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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