IOCAS-IR  > 实验海洋生物学重点实验室
Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
Wu, Songcui1,2,3; Gu, Wenhui1,2,3; Huang, Aiyou1,2,3; Li, Yuanxiang1,2,3; Kumar, Manoj4; Lim, Phaik Eem5; Huan, Li1,2,3; Gao, Shan1,2,3; Wang, Guangce1,2,3
2019-09-23
Source PublicationMICROBIAL CELL FACTORIES
Volume18Issue:1Pages:16
Corresponding AuthorWang, Guangce(gcwang@qdio.ac.cn)
AbstractBackground Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P. tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P. tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P. tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P. tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P. tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production.
KeywordGlucose-6-phosphate dehydrogenase Overexpression Antisense knockdown CO2 Phaeodactylum tricornutum Lipid accumulation Algal growth rate
DOI10.1186/s12934-019-1214-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41806171] ; National Natural Science Foundation of China[41876158] ; National Key R&D Program of China[2018YFD0901501-1] ; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811] ; Tianjin Marine Economic Demonstration[BHSF2017-21]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000487442400002
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162957
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Guangce
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.UTS, Fac Sci, Climate Change Cluster, Sydney, NSW, Australia
5.Univ Malaya, IOES, Kuala Lumpur 50603, Malaysia
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Songcui,Gu, Wenhui,Huang, Aiyou,et al. Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum[J]. MICROBIAL CELL FACTORIES,2019,18(1):16.
APA Wu, Songcui.,Gu, Wenhui.,Huang, Aiyou.,Li, Yuanxiang.,Kumar, Manoj.,...&Wang, Guangce.(2019).Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.MICROBIAL CELL FACTORIES,18(1),16.
MLA Wu, Songcui,et al."Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum".MICROBIAL CELL FACTORIES 18.1(2019):16.
Files in This Item:
File Name/Size DocType Version Access License
Wu-2019-Elevated-co-(2841KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Songcui]'s Articles
[Gu, Wenhui]'s Articles
[Huang, Aiyou]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Songcui]'s Articles
[Gu, Wenhui]'s Articles
[Huang, Aiyou]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Songcui]'s Articles
[Gu, Wenhui]'s Articles
[Huang, Aiyou]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Wu-2019-Elevated-co-improves-both-lipid-acc.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.