Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene

doi:10.1007/s10126-019-09911-z

IOCAS-IR > 实验海洋生物学重点实验室

	Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene
	Zhou, Li 1,2,3,4; Wang, Xueying1,2,3 ; Liu, Qinghua 1,2,3; Xu, Shihong 1,2,3; Zhao, Haixia 1,2,3,4; Han, Mingming 1,2,3; Wang, Yunong 1,2,3,4; Song, Zongcheng 5; Li, Jun 1,2,3
	2019-10-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	21 期号:5 页码:671-682
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	Primordial germ cells (PGCs) as the precursors of germ cells are responsible for transmitting genetic information to the next generation. Visualization of teleost PGCs in vivo is essential to research the origination and development of germ cells and facilitate further manipulation on PGCs isolation, cryopreservation, and surrogate breeding. In this study, artificially synthesized mRNAs constructed by fusing fluorescent protein coding region to the 3 ' untranslated region (3 ' UTR) of nanos3 or vasa (mCherry-Smnanos3 3 ' UTR or mCherry-Smvasa 3 ' UTR mRNA) were injected into turbot (Scophthalmus maximus) fertilized eggs for tracing PGCs. The results demonstrated that the fluorescent PGCs differentiated from somatic cells and aligned on both sides of the trunk at the early segmentation period, then migrated and located at the dorsal part of the gut where the gonad would form. In the same way, we also found that the zebrafish (Danio rerio) vasa 3 ' UTR could trace turbot PGCs, while the vasa 3 ' UTR s of marine medaka (Oryzias melastigma) and red seabream (Pagrus major) failed, although they could label the marine medaka PGCs. In addition, through comparative analysis, we discovered that some potential sequence elements in the3 ' UTRs of nanos3 and vasa, such as GCACs, 62-bp U-rich regions and nucleotide 187-218 regions might be involved in PGCs stabilization. The results of this study provided an efficient, rapid, and specific non-transgenic approach for visualizing PGCs of economical marine fish in vivo.
关键词	Primordial germ cells nanos3 vasa Scophthalmus maximus Location
DOI	10.1007/s10126-019-09911-z
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31572602] ; National Key Research and Development Program[2018YFD0901205] ; National Key Research and Development Program[2018YFD0901204] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0302-4] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; STS project[KFZD-SW-106] ; STS project[2017T3017] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470] ; National Natural Science Foundation of China[31572602] ; National Key Research and Development Program[2018YFD0901205] ; National Key Research and Development Program[2018YFD0901204] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0302-4] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; STS project[KFZD-SW-106] ; STS project[2017T3017] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000487127600009
出版者	SPRINGER
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162954
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Li,Wang, Xueying,Liu, Qinghua,et al. Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene[J]. MARINE BIOTECHNOLOGY,2019,21(5):671-682.
APA	Zhou, Li.,Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Zhao, Haixia.,...&Li, Jun.(2019).Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene.MARINE BIOTECHNOLOGY,21(5),671-682.
MLA	Zhou, Li,et al."Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene".MARINE BIOTECHNOLOGY 21.5(2019):671-682.