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Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene
Zhou, Li1,2,3,4; Wang, Xueying1,2,3; Liu, Qinghua1,2,3; Xu, Shihong1,2,3; Zhao, Haixia1,2,3,4; Han, Mingming1,2,3; Wang, Yunong1,2,3,4; Song, Zongcheng5; Li, Jun1,2,3
2019-10-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume21Issue:5Pages:671-682
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractPrimordial germ cells (PGCs) as the precursors of germ cells are responsible for transmitting genetic information to the next generation. Visualization of teleost PGCs in vivo is essential to research the origination and development of germ cells and facilitate further manipulation on PGCs isolation, cryopreservation, and surrogate breeding. In this study, artificially synthesized mRNAs constructed by fusing fluorescent protein coding region to the 3 ' untranslated region (3 ' UTR) of nanos3 or vasa (mCherry-Smnanos3 3 ' UTR or mCherry-Smvasa 3 ' UTR mRNA) were injected into turbot (Scophthalmus maximus) fertilized eggs for tracing PGCs. The results demonstrated that the fluorescent PGCs differentiated from somatic cells and aligned on both sides of the trunk at the early segmentation period, then migrated and located at the dorsal part of the gut where the gonad would form. In the same way, we also found that the zebrafish (Danio rerio) vasa 3 ' UTR could trace turbot PGCs, while the vasa 3 ' UTR s of marine medaka (Oryzias melastigma) and red seabream (Pagrus major) failed, although they could label the marine medaka PGCs. In addition, through comparative analysis, we discovered that some potential sequence elements in the3 ' UTRs of nanos3 and vasa, such as GCACs, 62-bp U-rich regions and nucleotide 187-218 regions might be involved in PGCs stabilization. The results of this study provided an efficient, rapid, and specific non-transgenic approach for visualizing PGCs of economical marine fish in vivo.
KeywordPrimordial germ cells nanos3 vasa Scophthalmus maximus Location
DOI10.1007/s10126-019-09911-z
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572602] ; National Key Research and Development Program[2018YFD0901205] ; National Key Research and Development Program[2018YFD0901204] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0302-4] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; STS project[KFZD-SW-106] ; STS project[2017T3017] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000487127600009
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162954
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Li,Wang, Xueying,Liu, Qinghua,et al. Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene[J]. MARINE BIOTECHNOLOGY,2019,21(5):671-682.
APA Zhou, Li.,Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Zhao, Haixia.,...&Li, Jun.(2019).Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene.MARINE BIOTECHNOLOGY,21(5),671-682.
MLA Zhou, Li,et al."Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene".MARINE BIOTECHNOLOGY 21.5(2019):671-682.
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