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The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila
Wang, Jingjing1,2; Yang, Bin2,3; Wang, Weilin4; Song, Xiaorui4; Jiang, Qiufen2; Qiu, Limei2; Wang, Lingling3,4,5; Song, Linsheng3,4,6
2019-08-28
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume10Pages:12
Corresponding AuthorSong, Linsheng(lshsong@dlou.edu.cn)
AbstractAccumulating evidences suggest that the enhanced immune responses and increased protection against bacteria-induced mortality can be initiated after the primary exposure to various microbial communities and their components in various organisms including commercially valuable crustaceans. In the present study, the survival rate and immune responses of Chinese mitten crab Eriocheir sinensis were determined after an immune priming (IP) with formalin-killed Aeromonas hydrophila and an immune challenge (ICH) with the same but live pathogen (Ah group). A group in which the animals received a salt injection prior to challenge was maintained as control (Ns group). In the present study, it was shown that an IP with killed A. hydrophila can significantly protect the crabs against the ICH with a lethal dose of the live pathogen. The increased survival was associated with elevated rate and duration of phagocytosis. The antibacterial activity of the serum was significantly increased in Ah group compared to that in Ns group. Significant changes of phenoloxidase (PO) activities were also found between Ah and Ns group but not in Ah group between IP and ICH. No significant changes of lysozyme were found in Ah and NS group during the whole experiment except 3 h after IP. In addition, the levels of transcripts and protein of Dscam were increased in hemocytes of the crabs from Ah group. All the results suggested that a primary immune priming with a particular killed pathogen could induce an enhanced immunity in crabs when they were encountered secondly with the same live pathogen. The evidences of elevated immune protections in crabs would contribute to better understand the mechanism of immune priming in invertebrates.
Keywordimmune priming Eriocheir sinensis Aeromonas hydrophila phagocytosis immune protection
DOI10.3389/fimmu.2019.02041
Indexed BySCI
Language英语
Funding ProjectNational Key RD Program[2018YFD0900606] ; National Natural Science Foundation of China[31530069] ; National Natural Science Foundation of China[31502202] ; AoShan Talents Cultivation Program by Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Shandong Province Natural Science Foundation[ZR2014CL035] ; Advanced Talents Foundation of Qingdao Agricultural University[6631114317] ; First class fishery discipline programme in Shandong Province, China ; Dalian High Level Talent Innovation Support Program[2015R020]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000482915500002
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162844
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
5.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian, Peoples R China
6.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Jingjing,Yang, Bin,Wang, Weilin,et al. The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila[J]. FRONTIERS IN IMMUNOLOGY,2019,10:12.
APA Wang, Jingjing.,Yang, Bin.,Wang, Weilin.,Song, Xiaorui.,Jiang, Qiufen.,...&Song, Linsheng.(2019).The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila.FRONTIERS IN IMMUNOLOGY,10,12.
MLA Wang, Jingjing,et al."The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila".FRONTIERS IN IMMUNOLOGY 10(2019):12.
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