The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila

doi:10.3389/fimmu.2019.02041

IOCAS-IR > 实验海洋生物学重点实验室

	The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila
	Wang, Jingjing1,2 ; Yang, Bin 2,3; Wang, Weilin4 ; Song, Xiaorui4 ; Jiang, Qiufen 2; Qiu, Limei 2; Wang, Lingling 3,4,5; Song, Linsheng 3,4,6
	2019-08-28
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	10 页码:12
通讯作者	Song, Linsheng(lshsong@dlou.edu.cn)
摘要	Accumulating evidences suggest that the enhanced immune responses and increased protection against bacteria-induced mortality can be initiated after the primary exposure to various microbial communities and their components in various organisms including commercially valuable crustaceans. In the present study, the survival rate and immune responses of Chinese mitten crab Eriocheir sinensis were determined after an immune priming (IP) with formalin-killed Aeromonas hydrophila and an immune challenge (ICH) with the same but live pathogen (Ah group). A group in which the animals received a salt injection prior to challenge was maintained as control (Ns group). In the present study, it was shown that an IP with killed A. hydrophila can significantly protect the crabs against the ICH with a lethal dose of the live pathogen. The increased survival was associated with elevated rate and duration of phagocytosis. The antibacterial activity of the serum was significantly increased in Ah group compared to that in Ns group. Significant changes of phenoloxidase (PO) activities were also found between Ah and Ns group but not in Ah group between IP and ICH. No significant changes of lysozyme were found in Ah and NS group during the whole experiment except 3 h after IP. In addition, the levels of transcripts and protein of Dscam were increased in hemocytes of the crabs from Ah group. All the results suggested that a primary immune priming with a particular killed pathogen could induce an enhanced immunity in crabs when they were encountered secondly with the same live pathogen. The evidences of elevated immune protections in crabs would contribute to better understand the mechanism of immune priming in invertebrates.
关键词	immune priming Eriocheir sinensis Aeromonas hydrophila phagocytosis immune protection
DOI	10.3389/fimmu.2019.02041
收录类别	SCI
语种	英语
资助项目	National Key RD Program[2018YFD0900606] ; National Natural Science Foundation of China[31530069] ; National Natural Science Foundation of China[31502202] ; AoShan Talents Cultivation Program by Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Shandong Province Natural Science Foundation[ZR2014CL035] ; Advanced Talents Foundation of Qingdao Agricultural University[6631114317] ; First class fishery discipline programme in Shandong Province, China ; Dalian High Level Talent Innovation Support Program[2015R020] ; National Key RD Program[2018YFD0900606] ; National Natural Science Foundation of China[31530069] ; National Natural Science Foundation of China[31502202] ; AoShan Talents Cultivation Program by Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Shandong Province Natural Science Foundation[ZR2014CL035] ; Advanced Talents Foundation of Qingdao Agricultural University[6631114317] ; First class fishery discipline programme in Shandong Province, China ; Dalian High Level Talent Innovation Support Program[2015R020]
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000482915500002
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162844
专题	实验海洋生物学重点实验室
通讯作者	Song, Linsheng
作者单位	1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China 5.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian, Peoples R China 6.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Jingjing,Yang, Bin,Wang, Weilin,et al. The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila[J]. FRONTIERS IN IMMUNOLOGY,2019,10:12.
APA	Wang, Jingjing.,Yang, Bin.,Wang, Weilin.,Song, Xiaorui.,Jiang, Qiufen.,...&Song, Linsheng.(2019).The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila.FRONTIERS IN IMMUNOLOGY,10,12.
MLA	Wang, Jingjing,et al."The Enhanced Immune Protection in Chinese Mitten Crab Eriocheir sinensis Against the Second Exposure to Bacteria Aeromonas hydrophila".FRONTIERS IN IMMUNOLOGY 10(2019):12.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
fimmu-10-02041.pdf（2588KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览