Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks

doi:10.3390/microorganisms7080250

	Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks
	Shang, Lixia 1,2,3; Hu, Zhangxi1,2,3 ; Deng, Yunyan 1,2,3; Liu, Yuyang 1,4; Zhai, Xinyu 1,4; Chai, Zhaoyang 1,2,3; Liu, Xiaohan 1,4; Zhan, Zifeng 5; Dobbs, Fred C.6; Tang, Ying Zhong 1,2,3
	2019-08-01
发表期刊	MICROORGANISMS
卷号	7 期号:8 页码:28
通讯作者	Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
摘要	Ships' ballast tanks have long been known as vectors for the introduction of organisms. We applied next-generation sequencing to detect dinoflagellates (mainly as cysts) in 32 ballast tank sediments collected during 2001-2003 from ships entering the Great Lakes or Chesapeake Bay and subsequently archived. Seventy-three dinoflagellates were fully identified to species level by this metagenomic approach and single-cell polymerase chain reaction (PCR)-based sequencing, including 19 toxic species, 36 harmful algal bloom (HAB) forming species, 22 previously unreported as producing cysts, and 55 reported from ballast tank sediments for the first time (including 13 freshwater species), plus 545 operational taxonomic units (OTUs) not fully identified due to a lack of reference sequences, indicating tank sediments are repositories of many previously undocumented taxa. Analyses indicated great heterogeneity of species composition among samples from different sources. Light and scanning electron microscopy and single-cell PCR sequencing supported and confirmed results of the metagenomic approach. This study increases the number of fully identified dinoflagellate species from ballast tank sediments to 142 (>50% increase). From the perspective of ballast water management, the high diversity and spatiotemporal heterogeneity of dinoflagellates in ballast tanks argues for continuing research and stringent adherence to procedures intended to prevent unintended introduction of non-indigenous toxic and HAB-forming species.
关键词	harmful algal blooms sediment metabarcoding invasive species dinoflagellate resting cysts Great Lakes Chesapeake Bay Margalefidinium polykrikoides single-cell PCR ballast water
DOI	10.3390/microorganisms7080250
收录类别	SCI
语种	英语
资助项目	NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Natural Science Foundation of China[41476142] ; National Natural Science Foundation of China[61533011] ; National Natural Science Foundation of China[41606126] ; National Natural Science Foundation of China[41506143] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Natural Science Foundation of China[41476142] ; National Natural Science Foundation of China[61533011] ; National Natural Science Foundation of China[41606126] ; National Natural Science Foundation of China[41506143] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000482956300034
出版者	MDPI
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162838
专题	海洋生态与环境科学重点实验室海洋生物分类与系统演化实验室
通讯作者	Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Shandong, Peoples R China 6.Old Dominion Univ, Dept Ocean Earth & Atmospher Sci, Norfolk, VA 23529 USA
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Shang, Lixia,Hu, Zhangxi,Deng, Yunyan,et al. Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks[J]. MICROORGANISMS,2019,7(8):28.
APA	Shang, Lixia.,Hu, Zhangxi.,Deng, Yunyan.,Liu, Yuyang.,Zhai, Xinyu.,...&Tang, Ying Zhong.(2019).Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks.MICROORGANISMS,7(8),28.
MLA	Shang, Lixia,et al."Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks".MICROORGANISMS 7.8(2019):28.