IOCAS-IR  > 海洋生态与环境科学重点实验室
Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks
Shang, Lixia1,2,3; Hu, Zhangxi1,2,3; Deng, Yunyan1,2,3; Liu, Yuyang1,4; Zhai, Xinyu1,4; Chai, Zhaoyang1,2,3; Liu, Xiaohan1,4; Zhan, Zifeng5; Dobbs, Fred C.6; Tang, Ying Zhong1,2,3
2019-08-01
Source PublicationMICROORGANISMS
Volume7Issue:8Pages:28
Corresponding AuthorTang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
AbstractShips' ballast tanks have long been known as vectors for the introduction of organisms. We applied next-generation sequencing to detect dinoflagellates (mainly as cysts) in 32 ballast tank sediments collected during 2001-2003 from ships entering the Great Lakes or Chesapeake Bay and subsequently archived. Seventy-three dinoflagellates were fully identified to species level by this metagenomic approach and single-cell polymerase chain reaction (PCR)-based sequencing, including 19 toxic species, 36 harmful algal bloom (HAB) forming species, 22 previously unreported as producing cysts, and 55 reported from ballast tank sediments for the first time (including 13 freshwater species), plus 545 operational taxonomic units (OTUs) not fully identified due to a lack of reference sequences, indicating tank sediments are repositories of many previously undocumented taxa. Analyses indicated great heterogeneity of species composition among samples from different sources. Light and scanning electron microscopy and single-cell PCR sequencing supported and confirmed results of the metagenomic approach. This study increases the number of fully identified dinoflagellate species from ballast tank sediments to 142 (>50% increase). From the perspective of ballast water management, the high diversity and spatiotemporal heterogeneity of dinoflagellates in ballast tanks argues for continuing research and stringent adherence to procedures intended to prevent unintended introduction of non-indigenous toxic and HAB-forming species.
Keywordharmful algal blooms sediment metabarcoding invasive species dinoflagellate resting cysts Great Lakes Chesapeake Bay Margalefidinium polykrikoides single-cell PCR ballast water
DOI10.3390/microorganisms7080250
Indexed BySCI
Language英语
Funding ProjectNSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Natural Science Foundation of China[41476142] ; National Natural Science Foundation of China[61533011] ; National Natural Science Foundation of China[41606126] ; National Natural Science Foundation of China[41506143] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000482956300034
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162838
Collection海洋生态与环境科学重点实验室
海洋生物分类与系统演化实验室
Corresponding AuthorTang, Ying Zhong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Shandong, Peoples R China
6.Old Dominion Univ, Dept Ocean Earth & Atmospher Sci, Norfolk, VA 23529 USA
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shang, Lixia,Hu, Zhangxi,Deng, Yunyan,et al. Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks[J]. MICROORGANISMS,2019,7(8):28.
APA Shang, Lixia.,Hu, Zhangxi.,Deng, Yunyan.,Liu, Yuyang.,Zhai, Xinyu.,...&Tang, Ying Zhong.(2019).Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks.MICROORGANISMS,7(8),28.
MLA Shang, Lixia,et al."Metagenomic Sequencing Identifies Highly Diverse Assemblages of Dinoflagellate Cysts in Sediments from Ships' Ballast Tanks".MICROORGANISMS 7.8(2019):28.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shang, Lixia]'s Articles
[Hu, Zhangxi]'s Articles
[Deng, Yunyan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shang, Lixia]'s Articles
[Hu, Zhangxi]'s Articles
[Deng, Yunyan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shang, Lixia]'s Articles
[Hu, Zhangxi]'s Articles
[Deng, Yunyan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.