Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis

doi:10.1111/j.1744-313X.2010.00954.x

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	Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis
	Li, F.1,2; Wang, L.1 ; Zhang, H.1 ; Zheng, P.1; Zhao, J.1 ; Qiu, L.1; Zhang, Y.1 ; Song, L.1 ; Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2010-12-01
发表期刊	INTERNATIONAL JOURNAL OF IMMUNOGENETICS
ISSN	1744-3121
卷号	37 期号:6 页码:499-508
文章类型	Article
摘要	P>NF-kappa B is a B-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. In the present study, a novel NF-kappa B-like transcription factor Relish was cloned from Chinese mitten crab Eriocheir sinensis (designated as EsRelish) by rapid amplification of cDNA ends (RACE) technique based on expressed sequence tag (EST). The full-length cDNA of EsRelish was of 5034 bp, consisting of a 5' untranslated region (UTR) of 57 bp, a 3' UTR of 1335 bp with two mRNA instability motifs (ATTTA), a polyadenylation signal sequence (AATAAA) and a poly (A) tail, and an open reading frame (ORF) of 3645 bp encoding a polypeptide of 1214 amino acids with a calculated molecular mass of 134.8 kDa and a theoretical isoelectric point of 5.26. There were a typical Rel homology domain (RHD), two nuclear localization signal (NLS) sequences (KR), an inhibitor kappa B (I kappa B)-like domain with six ankyrin repeats, a PEST region and a death domain in the deduced amino acid sequence of EsRelish. Conserved domain, higher similarity with other Rel/NF-kappa Bs and phylogenetic analysis suggested that EsRelish was a member of the NF-kappa B family. Quantitative real-time RT-PCR was employed to detect the mRNA transcripts of EsRelish in different tissues and its temporal expression in hemocytes of E. sinensis challenged with Pichia methanolica and Listonella anguillarum. The EsRelish mRNA was found to be constitutively expressed in a wide range of tissues. It could be mainly detected in the hemocytes, gonad and hepatopancreas, and less degree in the gill, muscle and heart. The expression level of EsRelish mRNA in hemocytes was up-regulated from at 3, 6, 9 and 12 h after P. methanolica challenge. In L. anguillarum challenge, it was up-regulated at 9, 12 and 24 h. The results collectively indicated that EsRelish was potentially involved in the immune response against fungus and bacteria.; P>NF-kappa B is a B-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. In the present study, a novel NF-kappa B-like transcription factor Relish was cloned from Chinese mitten crab Eriocheir sinensis (designated as EsRelish) by rapid amplification of cDNA ends (RACE) technique based on expressed sequence tag (EST). The full-length cDNA of EsRelish was of 5034 bp, consisting of a 5' untranslated region (UTR) of 57 bp, a 3' UTR of 1335 bp with two mRNA instability motifs (ATTTA), a polyadenylation signal sequence (AATAAA) and a poly (A) tail, and an open reading frame (ORF) of 3645 bp encoding a polypeptide of 1214 amino acids with a calculated molecular mass of 134.8 kDa and a theoretical isoelectric point of 5.26. There were a typical Rel homology domain (RHD), two nuclear localization signal (NLS) sequences (KR), an inhibitor kappa B (I kappa B)-like domain with six ankyrin repeats, a PEST region and a death domain in the deduced amino acid sequence of EsRelish. Conserved domain, higher similarity with other Rel/NF-kappa Bs and phylogenetic analysis suggested that EsRelish was a member of the NF-kappa B family. Quantitative real-time RT-PCR was employed to detect the mRNA transcripts of EsRelish in different tissues and its temporal expression in hemocytes of E. sinensis challenged with Pichia methanolica and Listonella anguillarum. The EsRelish mRNA was found to be constitutively expressed in a wide range of tissues. It could be mainly detected in the hemocytes, gonad and hepatopancreas, and less degree in the gill, muscle and heart. The expression level of EsRelish mRNA in hemocytes was up-regulated from at 3, 6, 9 and 12 h after P. methanolica challenge. In L. anguillarum challenge, it was up-regulated at 9, 12 and 24 h. The results collectively indicated that EsRelish was potentially involved in the immune response against fungus and bacteria.
关键词	Nf-kappa-b Innate Immune-response Mosquito Aedes-aegypti Spot Syndrome Virus Tremor Disease Transcription Factors Pathogen Infection Dna-binding Drosophila Activation
学科领域	Genetics & Heredity ; Immunology
DOI	10.1111/j.1744-313X.2010.00954.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000283373500010
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1627
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室海洋环境腐蚀与生物污损重点实验室
通讯作者	Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, F.,Wang, L.,Zhang, H.,et al. Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis[J]. INTERNATIONAL JOURNAL OF IMMUNOGENETICS,2010,37(6):499-508.
APA	Li, F..,Wang, L..,Zhang, H..,Zheng, P..,Zhao, J..,...&Wang, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis.INTERNATIONAL JOURNAL OF IMMUNOGENETICS,37(6),499-508.
MLA	Li, F.,et al."Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis".INTERNATIONAL JOURNAL OF IMMUNOGENETICS 37.6(2010):499-508.

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