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The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas
Du, Yang1,2; Wang, Mengqiang1,2; Wang, Baojie1,2; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Lei1,2,3
2019-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume92Pages:119-124
Corresponding AuthorWang, Lei(wanglei@qdio.ac.cn)
AbstractOur previous work showed that Lactobacillus pentosus HC-2 has high antibacterial and adhesion activity, and as a probiotic could improve the nutrients and immunomodulatory effects in the Litopenaeus vannamei farming. In order to further investigate the influence of HC-2 surface protein on its probiotic effects, the immune and digestion related genes expression and enzymes activities, the colonization numbers of HC-2, and the histologic characteristics were analysis in shrimp hepatopancreas after feeding either the intact surface proteins, the probiotic treated with lithium chloride (LiCl) to remove noncovalently bound surface proteins or no probiotic for four weeks. The results showed that the immune genes expression of lysozyme, proPO, LGBP, Penaeidins-3 alpha, crustin and C-type lectin, the immune enzymes activities of superoxide dismutase, catalase and Alkaline phosphatase, and the digestion enzymes of Trypsin, Lipase and alpha-Amylase were significantly higher in hepatopancreas of shrimp fed with intact HC-2 than that in shrimp fed with base diet or striped surface proteins HC-2 post feeding and challenge. In addition, the shrimp fed with intact HC-2 leads to the bacteria cells adhesion to hepatopancreas was significantly higher than that in shrimp fed with no surface proteins HC-2. Furthermore, the tissue damages of hepatopancreas caused by pathogenic vibrio were obviously observed in shrimp fed with base diet or no surface proteins HC-2, but no signs of damages were found in shrimp fed with intact HC-2. These results demonstrate that surface proteins are important components for HC-2 to execute probiotic effect that improve hepatopancreas immune response and nutrition digestion to protect shrimp against pathogen damage.
KeywordProbiotic Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins
DOI10.1016/j.fsi.2019.06.003
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2018M632736] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000483634300015
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162739
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Yang,Wang, Mengqiang,Wang, Baojie,et al. The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92:119-124.
APA Du, Yang,Wang, Mengqiang,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2019).The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas.FISH & SHELLFISH IMMUNOLOGY,92,119-124.
MLA Du, Yang,et al."The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas".FISH & SHELLFISH IMMUNOLOGY 92(2019):119-124.
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