Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas | |
Du, Yang1,2; Wang, Mengqiang1,2 ; Wang, Baojie1,2; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Lei1,2,3
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| 2019-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 92页码:119-124 |
| 通讯作者 | Wang, Lei(wanglei@qdio.ac.cn) |
| 摘要 | Our previous work showed that Lactobacillus pentosus HC-2 has high antibacterial and adhesion activity, and as a probiotic could improve the nutrients and immunomodulatory effects in the Litopenaeus vannamei farming. In order to further investigate the influence of HC-2 surface protein on its probiotic effects, the immune and digestion related genes expression and enzymes activities, the colonization numbers of HC-2, and the histologic characteristics were analysis in shrimp hepatopancreas after feeding either the intact surface proteins, the probiotic treated with lithium chloride (LiCl) to remove noncovalently bound surface proteins or no probiotic for four weeks. The results showed that the immune genes expression of lysozyme, proPO, LGBP, Penaeidins-3 alpha, crustin and C-type lectin, the immune enzymes activities of superoxide dismutase, catalase and Alkaline phosphatase, and the digestion enzymes of Trypsin, Lipase and alpha-Amylase were significantly higher in hepatopancreas of shrimp fed with intact HC-2 than that in shrimp fed with base diet or striped surface proteins HC-2 post feeding and challenge. In addition, the shrimp fed with intact HC-2 leads to the bacteria cells adhesion to hepatopancreas was significantly higher than that in shrimp fed with no surface proteins HC-2. Furthermore, the tissue damages of hepatopancreas caused by pathogenic vibrio were obviously observed in shrimp fed with base diet or no surface proteins HC-2, but no signs of damages were found in shrimp fed with intact HC-2. These results demonstrate that surface proteins are important components for HC-2 to execute probiotic effect that improve hepatopancreas immune response and nutrition digestion to protect shrimp against pathogen damage. |
| 关键词 | Probiotic Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins |
| DOI | 10.1016/j.fsi.2019.06.003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | China Postdoctoral Science Foundation[2018M632736] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] ; China Postdoctoral Science Foundation[2018M632736] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000483634300015 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/162739 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Du, Yang,Wang, Mengqiang,Wang, Baojie,et al. The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92:119-124. |
| APA | Du, Yang,Wang, Mengqiang,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2019).The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas.FISH & SHELLFISH IMMUNOLOGY,92,119-124. |
| MLA | Du, Yang,et al."The influence of surface proteins on the probiotic effects of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei hepatopancreas".FISH & SHELLFISH IMMUNOLOGY 92(2019):119-124. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Du-2019-The-influenc（1685KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
| The influence of sur（1686KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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