Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis | |
Su, Jingqian1; Guo, Kai1; Huang, Min1; Liu, Yixuan1; Zhang, Jie2 ; Sun, Lijun1,3; Li, Daliang1; Pang, Ka-Lai4,5; Wang, Guangce6,7; Chen, Long8; Liu, Zhiyu9; Chen, Youqiang2; Chen, Qi1; Huang, Luqiang2
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| 2019-08-28 | |
| Source Publication | FRONTIERS IN PHARMACOLOGY
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| ISSN | 1663-9812 |
| Volume | 10Pages:17 |
| Corresponding Author | Chen, Youqiang(yqchen@fjnu.edu.cn) ; Chen, Qi(nfsw@fjnu.edu.cn) ; Huang, Luqiang(biohlq@fjnu.edu.cn) |
| Abstract | Background: Fucoxanthin (FX), a xanthophyll pigment which occurs in marine brown algae with remarkable biological properties, has been proven to be safe for consumption by animals. Although FX has various pharmacological effects including anti-inflammatory, anti-tumor, anti-obesity, antioxidant, anti-diabetic, anti-malarial, and anti-lipid, in vivo protective effect against sepsis has not been reported. In this study, we aimed at evaluation the efficacy of the FX in a model of sepsis mouse. Methods: FX was successfully isolated from Conticribra weissflogii ND-8 for the first time. The FX was identified by thin-layer chromatography (TLC), high-performance liquid chromatography-mass spectrometry (HPLC-MS), and nuclear magnetic resonance (NMR). Animals were randomly divided into 9 groups, including Sham group (mouse received an intraperitoneal injection of normal saline 1.0 ml/kg), FX-treated (0.1-1.0 ml/kg), Lipopolysaccharide (LPS)-treated (20 mg/kg), FX+LPS-treated (0.1-10.0 mg/kg and 20 mg/kg, respectively), and urinastatin groups (10(4) U/kg). Nuclear factor (NF)-kappa B activation could be potential treatment for sepsis. NF-kappa B signaling components were determined by western-blotting. IL-6, IL-1 beta, TNF-alpha production, and NF-kappa B activation were evaluated by ELISA and immunofluorescent staining in vitro. Results: FX was found to decrease the expression of inflammatory cytokines including IL-6, IL-1 beta, and TNF-alpha, in a prophylactic manner in the LPS-induced sepsis mouse model. Meanwhile, FX significantly inhibits phosphorylation of the NF-kappa B signaling pathway induced by LPS at the cellular level and reduces the nuclear translocation of NF-kappa B. The IC50 for suppressing the expression of NF-kappa B was 11.08 +/- 0.78 mu M in the THP1-Lucia (TM) NF-kappa B cells. Furthermore, FX also inhibits the expression of inflammatory factors in a dose-dependent manner with the IC50 inhibition of IL-6 production was 2.19 +/- 0.70 mu M in Raw267.4 macrophage cells. It is likely that the molecules with the ability of targeting NF-kappa B activation and inflammasome assembly, such as fucoxanthin, are interesting subjects to be used for treating sepsis. |
| Keyword | fucoxanthin lipopolysaccharide nuclear factor-kappa B sepsis Conticribra weissflogii |
| DOI | 10.3389/fphar.2019.00906 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[81803547] ; Natural Science Foundation of Fujian Province, China[2018J01720] ; Innovative Research Teams Program II of Fujian Normal University in China[IRTL1703] ; Innovative Research Teams Program II of Fujian Normal University in China[FZSKG2018013] ; Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration ; Provincial University Project of Fujian Education Department[JK2017020] |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Pharmacology & Pharmacy |
| WOS ID | WOS:000482910300001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/162730 |
| Collection | 中国科学院海洋研究所 |
| Corresponding Author | Chen, Youqiang; Chen, Qi; Huang, Luqiang |
| Affiliation | 1.Fujian Normal Univ, Coll Life Sci, Biomed Res Ctr South China, Fujian Key Lab Innate Immune Biol, Fuzhou, Fujian, Peoples R China 2.Fujian Normal Univ, Publ Serv Platform Ind Dev Technol Marine Biol Me, Ctr Engn Technol Res Microalgae Germplasm Improve, Southern Inst Oceanog, Fuzhou, Fujian, Peoples R China 3.Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA 4.Natl Taiwan Ocean Univ, Inst Marine Biol, Keelung, Taiwan 5.Natl Taiwan Ocean Univ, Ctr Excellence Oceans, Keelung, Taiwan 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 8.Fudan Univ, Huashan Hosp, Div Neurocrit Care, Shanghai, Peoples R China 9.Fisheries Res Inst Fujian, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Key Lab Cultivat & High value Utilizat Marine Org, Xiamen, Fujian, Peoples R China |
| Recommended Citation GB/T 7714 | Su, Jingqian,Guo, Kai,Huang, Min,et al. Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis[J]. FRONTIERS IN PHARMACOLOGY,2019,10:17. |
| APA | Su, Jingqian.,Guo, Kai.,Huang, Min.,Liu, Yixuan.,Zhang, Jie.,...&Huang, Luqiang.(2019).Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis.FRONTIERS IN PHARMACOLOGY,10,17. |
| MLA | Su, Jingqian,et al."Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis".FRONTIERS IN PHARMACOLOGY 10(2019):17. |
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