Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis

doi:10.3389/fphar.2019.00906

IOCAS-IR

	Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis
	Su, Jingqian 1; Guo, Kai 1; Huang, Min 1; Liu, Yixuan 1; Zhang, Jie2 ; Sun, Lijun 1,3; Li, Daliang 1; Pang, Ka-Lai 4,5; Wang, Guangce 6,7; Chen, Long 8; Liu, Zhiyu 9; Chen, Youqiang 2; Chen, Qi 1; Huang, Luqiang 2
	2019-08-28
Source Publication	FRONTIERS IN PHARMACOLOGY
ISSN	1663-9812
Volume	10 Pages:17
Corresponding Author	Chen, Youqiang(yqchen@fjnu.edu.cn) ; Chen, Qi(nfsw@fjnu.edu.cn) ; Huang, Luqiang(biohlq@fjnu.edu.cn)
Abstract	Background: Fucoxanthin (FX), a xanthophyll pigment which occurs in marine brown algae with remarkable biological properties, has been proven to be safe for consumption by animals. Although FX has various pharmacological effects including anti-inflammatory, anti-tumor, anti-obesity, antioxidant, anti-diabetic, anti-malarial, and anti-lipid, in vivo protective effect against sepsis has not been reported. In this study, we aimed at evaluation the efficacy of the FX in a model of sepsis mouse. Methods: FX was successfully isolated from Conticribra weissflogii ND-8 for the first time. The FX was identified by thin-layer chromatography (TLC), high-performance liquid chromatography-mass spectrometry (HPLC-MS), and nuclear magnetic resonance (NMR). Animals were randomly divided into 9 groups, including Sham group (mouse received an intraperitoneal injection of normal saline 1.0 ml/kg), FX-treated (0.1-1.0 ml/kg), Lipopolysaccharide (LPS)-treated (20 mg/kg), FX+LPS-treated (0.1-10.0 mg/kg and 20 mg/kg, respectively), and urinastatin groups (10(4) U/kg). Nuclear factor (NF)-kappa B activation could be potential treatment for sepsis. NF-kappa B signaling components were determined by western-blotting. IL-6, IL-1 beta, TNF-alpha production, and NF-kappa B activation were evaluated by ELISA and immunofluorescent staining in vitro. Results: FX was found to decrease the expression of inflammatory cytokines including IL-6, IL-1 beta, and TNF-alpha, in a prophylactic manner in the LPS-induced sepsis mouse model. Meanwhile, FX significantly inhibits phosphorylation of the NF-kappa B signaling pathway induced by LPS at the cellular level and reduces the nuclear translocation of NF-kappa B. The IC50 for suppressing the expression of NF-kappa B was 11.08 +/- 0.78 mu M in the THP1-Lucia (TM) NF-kappa B cells. Furthermore, FX also inhibits the expression of inflammatory factors in a dose-dependent manner with the IC50 inhibition of IL-6 production was 2.19 +/- 0.70 mu M in Raw267.4 macrophage cells. It is likely that the molecules with the ability of targeting NF-kappa B activation and inflammasome assembly, such as fucoxanthin, are interesting subjects to be used for treating sepsis.
Keyword	fucoxanthin lipopolysaccharide nuclear factor-kappa B sepsis Conticribra weissflogii
DOI	10.3389/fphar.2019.00906
Indexed By	SCI
Language	英语
Funding Project	National Natural Science Foundation of China[81803547] ; Natural Science Foundation of Fujian Province, China[2018J01720] ; Innovative Research Teams Program II of Fujian Normal University in China[IRTL1703] ; Innovative Research Teams Program II of Fujian Normal University in China[FZSKG2018013] ; Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration ; Provincial University Project of Fujian Education Department[JK2017020] ; National Natural Science Foundation of China[81803547] ; Natural Science Foundation of Fujian Province, China[2018J01720] ; Innovative Research Teams Program II of Fujian Normal University in China[IRTL1703] ; Innovative Research Teams Program II of Fujian Normal University in China[FZSKG2018013] ; Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration ; Provincial University Project of Fujian Education Department[JK2017020]
WOS Research Area	Pharmacology & Pharmacy
WOS Subject	Pharmacology & Pharmacy
WOS ID	WOS:000482910300001
Publisher	FRONTIERS MEDIA SA
Citation statistics	Cited Times:10[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.qdio.ac.cn/handle/337002/162730
Collection	中国科学院海洋研究所
Corresponding Author	Chen, Youqiang; Chen, Qi; Huang, Luqiang
Affiliation	1.Fujian Normal Univ, Coll Life Sci, Biomed Res Ctr South China, Fujian Key Lab Innate Immune Biol, Fuzhou, Fujian, Peoples R China 2.Fujian Normal Univ, Publ Serv Platform Ind Dev Technol Marine Biol Me, Ctr Engn Technol Res Microalgae Germplasm Improve, Southern Inst Oceanog, Fuzhou, Fujian, Peoples R China 3.Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA 4.Natl Taiwan Ocean Univ, Inst Marine Biol, Keelung, Taiwan 5.Natl Taiwan Ocean Univ, Ctr Excellence Oceans, Keelung, Taiwan 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 8.Fudan Univ, Huashan Hosp, Div Neurocrit Care, Shanghai, Peoples R China 9.Fisheries Res Inst Fujian, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Key Lab Cultivat & High value Utilizat Marine Org, Xiamen, Fujian, Peoples R China
Recommended Citation GB/T 7714	Su, Jingqian,Guo, Kai,Huang, Min,et al. Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis[J]. FRONTIERS IN PHARMACOLOGY,2019,10:17.
APA	Su, Jingqian.,Guo, Kai.,Huang, Min.,Liu, Yixuan.,Zhang, Jie.,...&Huang, Luqiang.(2019).Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis.FRONTIERS IN PHARMACOLOGY,10,17.
MLA	Su, Jingqian,et al."Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis".FRONTIERS IN PHARMACOLOGY 10(2019):17.

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