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A single-CRD C-type lectin (CgCLec-3) with novel DIN motif exhibits versatile immune functions in Crassostrea gigas
Song, Xiaorui1; Xin, Xiaoyu1; Wang, Hao3; Li, Hui3; Zhang, Huan3; Jia, Zhihao3; Liu, Conghui3; Jiang, Shuai3; Wang, Lingling2,4; Song, Linsheng1,2,4
2019-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume92Pages:772-781
Corresponding AuthorSong, Linsheng(lshsong@dlou.edu.cn)
AbstractC-type lectins (CTLs), as important pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins which participate in nonself-recognition and eliminating pathogens. In the present study, a novel CTL (designated as CgCLec-3) was identified from the Pacific oyster Crassostrea gigas. There was only one carbohydrate-recognition domain (CRD) of 151 amino acid residues within the deduced amino acid sequence of CgCLec-3. The deduced amino acid sequence of CgCLec-3 CRD shared low homology with the CRDs of other CTLs in oyster with the identities ranging from 12% to 22%. A novel DIN motif was found in Ca2+-binding site 2 of CgCLec-3. The relative expression level of CgCLec-3 in hemocytes was up-regulated significantly after the stimulations of bacteria and Pathogen Associated Molecular Patterns (PAMPs). Irnmunohistochemistry assay showed that CgCLec-3 protein was mainly distributed in gill and mantle, less in gonad, and could not be detected in adductor muscle and hepatopancreas. The recombinant protein (rCgCLec-3) could bind lipopolysaccharide (LPS), mannose (MAN) and peptidoglycan (PGN), but not poly (I:C). rCgCLec-3 odhibited strong binding ability to Vibrio anguillartun and V. splendidus, moderate binding activities to Escherichia colt, Pichia pastoris and Yatrowia lipotica, weak binding affinity to Staphylococcus aureus and Mictvcoccus luteus. rCgCLec-3 could agglutinate microorganisms, in a Ca2+-dependent manner and its activity to agglutinate V. splendidus was remarkably higher than that to agglutinate E. coli, S. aureus and P. pastoris. The phagocytic activity of oyster hemocytes was significantly enhanced after incubation with rCgCLec-3. rCgCLec-3 also exhibited antibacterial activity against E. coli and S. aureus. The results clearly suggested that CgCLec-3 in Pacific oyster C. gigas not only served as a PRR involved in the PAMPs recognition and microbes binding, but also functioned as an immune effector participating in the clearance of invaders.
KeywordCrassostrea gigas C-type lectin DIN motif PAMP binding Agglutination Antibacterial activity
DOI10.1016/j.fsi.2019.07.001
Indexed BySCI
Language英语
Funding ProjectNational key R&D Program of China[2018YFD0900502] ; National Science Foundation of China[U1706204] ; Distinguished Professor of Liaoning, Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Aoshan Talent Cultivation Program - Qingdao National Laboratory for Marine Science ; Earmarked Fund from Modem Agro-industry Technology Research System[CARS-49] ; Natural Science Foundation of Liaoning, China[20170520056] ; Technology and Talented Scholars in Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000483634300084
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162721
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Song, Xiaorui,Xin, Xiaoyu,Wang, Hao,et al. A single-CRD C-type lectin (CgCLec-3) with novel DIN motif exhibits versatile immune functions in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92:772-781.
APA Song, Xiaorui.,Xin, Xiaoyu.,Wang, Hao.,Li, Hui.,Zhang, Huan.,...&Song, Linsheng.(2019).A single-CRD C-type lectin (CgCLec-3) with novel DIN motif exhibits versatile immune functions in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,92,772-781.
MLA Song, Xiaorui,et al."A single-CRD C-type lectin (CgCLec-3) with novel DIN motif exhibits versatile immune functions in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 92(2019):772-781.
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