Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress | |
Liu, Youli1,2,3; Li, Li1,4,5,6; Huang, Baoyu1,4,5,6 ; Wang, Wei1,2,5,6; Zhang, Guofan1,2,5,6
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| 2019-08-08 | |
| Source Publication | BMC GENOMICS
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| ISSN | 1471-2164 |
| Volume | 20Issue:1Pages:15 |
| Corresponding Author | Li, Li(lili@qdio.ac.cn) |
| Abstract | Background The Pacific oyster Crassostrea gigas is an important fishery resource that is sensitive to temperature fluctuations. Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. In other animals, heat shock response is a transcriptional response driven by the heat shock transcription factor 1 (HSF1) and thermal stress can trigger HSP70 expression to protect the organism via HSF1. However, the regulatory relationship between HSF1 and HSP remains unclear in Pacific oyster. Therefore, in the present study, we examined the transcriptomic response of several to thermal stress following HSF1 interference. Results We identified 150 genes responsive to heat shock including seven HSP genes, six of which belonging to the group of 17 HSP genes enriched in response to heat shock, according to weighted gene co-expression network analysis (WGCNA). The other gene was enriched in the module correlated with HSF1 interference. In addition, we found 48 and 47 genes that were upregulated and downregulated by HSF1 in response to heat shock, respectively. In the upregulated genes, we identified one HSP70 potentially regulated by HSF1 in response to heat shock. Furthermore, based on differentially expressed genes and WGCNA analyses, we found that the hypoxia signaling pathway was enriched under heat shock conditions. Five genes were then selected to detect dynamic changes through time. The results suggested that gene expression was correlated with HSF1 expression. The regulation of HSP70 by HSF1 was preliminarily confirmed by binding site predictions and by a dual luciferase assay. Conclusions Our results revealed that the expression of HSP70 and HSP20 was initially triggered after 2 h of heat shock, and one of the HSP70 genes was potentially regulated by HSF1. From these results, it is evident that not all heat-inducible genes were triggered simultaneously in response to heat shock stress. Overall, the results revealed a possible HSF1-HSP regulatory relationship in Pacific oyster, providing valuable information on the mechanisms of thermal tolerance in this commercially important oyster. |
| Keyword | Crassostrea gigas Heat shock HSP HSF1 Regulatory RNA interference Transcriptome WGCNA |
| DOI | 10.1186/s12864-019-6003-8 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFD0900304] ; National Natural Science Fundation of China[31572620] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS Subject | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS ID | WOS:000480474900002 |
| Publisher | BMC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/162397 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Li, Li |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, Youli,Li, Li,Huang, Baoyu,et al. RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress[J]. BMC GENOMICS,2019,20(1):15. |
| APA | Liu, Youli,Li, Li,Huang, Baoyu,Wang, Wei,&Zhang, Guofan.(2019).RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress.BMC GENOMICS,20(1),15. |
| MLA | Liu, Youli,et al."RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress".BMC GENOMICS 20.1(2019):15. |
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