The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization

doi:10.1007/s10811-019-1740-2

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	The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization
	Zou, Yaxue 1; Fu, Xiaoting 1; Liu, Nannan 2; Duan, Delin 2,3; Wang, Xueliang 4; Xu, Jiachao1 ; Gao, Xin 1
	2019-08-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	31 期号:4 页码:2547-2558
通讯作者	Fu, Xiaoting(xiaotingfu@ouc.edu.cn)
摘要	The anti-inflammatory activities of agaro-oligosaccharide (AOS) fractions with different degrees of polymerization (DPs) were compared, and their anti-inflammatory mechanisms were investigated. Total AOS (F0) was prepared from the agar of Gracilaria lemaneiformis, and its three separated fractions of high DP (F1), medium DP (F2), and low DP (F3) were obtained using Bio-Gel P2 chromatography. F2 showed a high anti-inflammatory activity but also a potential cytotoxicity, while F1 and F3 showed no cytotoxicity but weak anti-inflammatory activities. The total AOS fractions of F0 exhibited a higher anti-inflammatory activity than the summarized activities of the separated fractions; moreover, the combination of the three fractions significantly alleviated the potential cytotoxicity of F2. Thus, the synergistic anti-inflammatory effects of the AOS fractions were revealed. Furthermore, the anti-inflammatory mechanisms of F0 were investigated both in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro and LPS-induced zebrafish embryos in vivo. F0 inhibited NO production and PGE2 expression via the downregulation of iNOS and COX-2 expression, and it suppressed TNF-alpha, IL-1 beta, and IL-6 productions at the protein and mRNA levels in RAW 264.7 cells. It is the first time that the zebrafish model was used for the determination of anti-inflammatory activities of AOS, and F0 showed to suppress the productions of NO and ROS by exerting a protective effect against the phenotypic changes and the cell damage caused by LPS. This is the first report of the synergistic anti-inflammatory activities of AOS fractions. The result revealed that AOS without separation and purification showed a favorable anti-inflammatory activity, which was easy to be produced and had potential application in the food, pharmaceutical, and cosmetic industries.
关键词	Rhodophyta Agaro-oligosaccharide Anti-inflammation RAW 264.7 Zebrafish
DOI	10.1007/s10811-019-1740-2
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901104] ; International Science & Technology Cooperation Program of China[2015DFG32290] ; Jiangsu Provincial Key RD Project[BE2015335] ; National Key R&D Program of China[2018YFD0901104] ; International Science & Technology Cooperation Program of China[2015DFG32290] ; Jiangsu Provincial Key RD project[BE2015335]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000479057200035
出版者	SPRINGER
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162385
专题	实验海洋生物学重点实验室
通讯作者	Fu, Xiaoting
作者单位	1.Ocean Univ China, Coll Food Sci & Engn, 5th Yushan Rd, Qingdao 266003, Shandong, Peoples R China 2.State Key Lab Seaweed Bioact Subst, 1th Daxueyuan Rd, Qingdao 266400, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7th Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Fujian Global Ocean Biotechnol Co Ltd, Downtown Marine Biomed Zhaoan Fujian High Tech In, Zhangzhou 363502, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Zou, Yaxue,Fu, Xiaoting,Liu, Nannan,et al. The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2547-2558.
APA	Zou, Yaxue.,Fu, Xiaoting.,Liu, Nannan.,Duan, Delin.,Wang, Xueliang.,...&Gao, Xin.(2019).The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization.JOURNAL OF APPLIED PHYCOLOGY,31(4),2547-2558.
MLA	Zou, Yaxue,et al."The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2547-2558.

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