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The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization
Zou, Yaxue1; Fu, Xiaoting1; Liu, Nannan2; Duan, Delin2,3; Wang, Xueliang4; Xu, Jiachao1; Gao, Xin1
2019-08-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume31Issue:4Pages:2547-2558
Corresponding AuthorFu, Xiaoting(xiaotingfu@ouc.edu.cn)
AbstractThe anti-inflammatory activities of agaro-oligosaccharide (AOS) fractions with different degrees of polymerization (DPs) were compared, and their anti-inflammatory mechanisms were investigated. Total AOS (F0) was prepared from the agar of Gracilaria lemaneiformis, and its three separated fractions of high DP (F1), medium DP (F2), and low DP (F3) were obtained using Bio-Gel P2 chromatography. F2 showed a high anti-inflammatory activity but also a potential cytotoxicity, while F1 and F3 showed no cytotoxicity but weak anti-inflammatory activities. The total AOS fractions of F0 exhibited a higher anti-inflammatory activity than the summarized activities of the separated fractions; moreover, the combination of the three fractions significantly alleviated the potential cytotoxicity of F2. Thus, the synergistic anti-inflammatory effects of the AOS fractions were revealed. Furthermore, the anti-inflammatory mechanisms of F0 were investigated both in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro and LPS-induced zebrafish embryos in vivo. F0 inhibited NO production and PGE2 expression via the downregulation of iNOS and COX-2 expression, and it suppressed TNF-alpha, IL-1 beta, and IL-6 productions at the protein and mRNA levels in RAW 264.7 cells. It is the first time that the zebrafish model was used for the determination of anti-inflammatory activities of AOS, and F0 showed to suppress the productions of NO and ROS by exerting a protective effect against the phenotypic changes and the cell damage caused by LPS. This is the first report of the synergistic anti-inflammatory activities of AOS fractions. The result revealed that AOS without separation and purification showed a favorable anti-inflammatory activity, which was easy to be produced and had potential application in the food, pharmaceutical, and cosmetic industries.
KeywordRhodophyta Agaro-oligosaccharide Anti-inflammation RAW 264.7 Zebrafish
DOI10.1007/s10811-019-1740-2
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0901104] ; International Science & Technology Cooperation Program of China[2015DFG32290] ; Jiangsu Provincial Key RD project[BE2015335]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000479057200035
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162385
Collection实验海洋生物学重点实验室
Corresponding AuthorFu, Xiaoting
Affiliation1.Ocean Univ China, Coll Food Sci & Engn, 5th Yushan Rd, Qingdao 266003, Shandong, Peoples R China
2.State Key Lab Seaweed Bioact Subst, 1th Daxueyuan Rd, Qingdao 266400, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7th Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Fujian Global Ocean Biotechnol Co Ltd, Downtown Marine Biomed Zhaoan Fujian High Tech In, Zhangzhou 363502, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Zou, Yaxue,Fu, Xiaoting,Liu, Nannan,et al. The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2547-2558.
APA Zou, Yaxue.,Fu, Xiaoting.,Liu, Nannan.,Duan, Delin.,Wang, Xueliang.,...&Gao, Xin.(2019).The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization.JOURNAL OF APPLIED PHYCOLOGY,31(4),2547-2558.
MLA Zou, Yaxue,et al."The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2547-2558.
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