Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells | |
Liu, YingJuan1,2,3; Deng, Zhenzhen1,2,3; Geng, Lihua1,2,3; Wang, Jing1,2 ; Zhang, Quanbin1,2
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| 2019-08-01 | |
| Source Publication | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| Volume | 31Issue:4Pages:2559-2571 |
| Corresponding Author | Wang, Jing(jingwang@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| Abstract | Porphyran, derived from the marine red algae Porphyra and Pyropia, is a type of polysaccharide. Oligo-porphyran (OP) was prepared from porphyran (isolated from Pyropia yezoensis) through the acidolysis reaction containing a linear backbone of alternating 3-linked beta-d-galactose and 4-linked alpha-l-galactose-6-sulfate. Our previous studies have shown that oligo-porphyran could attenuate MPTP-induced behavioral deficits in mice by inhibiting the tyrosine hydroxylase (TH) loss. In the present study, we further investigated how OP protects the dopamine neurons from loss in PC12 cells. OP significantly attenuated LDH release and the production of reactive oxygen species (ROS) lesioned by 6-OHDA. Moreover, OP alleviated the mitochondrial membrane potential (MMP) loss, the ratio of Bax/Bcl-2, and upregulated the PI3K/ Akt/PKC pathway, the level of tyrosine hydroxylase (TH), and dopamine transporter (DAT). Furthermore, OP decreased the production of proinflammatory cytokines. Terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining further indicated that OP treatment inhibited apoptosis. These results might contribute to the overall understanding of the potential health benefits of OP for food and drug applications. |
| Keyword | Rhodophyta Oligo-porphyran Apoptosis 6-OHDA PC12 cells Mitochondria |
| DOI | 10.1007/s10811-018-1713-x |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key RD Plan[2018YFD0901104] ; Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of the Chinese Academy of Sciences[ZSTH-025] ; Natural Science Foundation of China[41406144] ; Natural Science Foundation of China[41506165] ; Ocean Public Welfare Scientific Research Project ; State Oceanic Administration of the People's Republic of China[201405040] ; Science and Technology project of Shandong province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS Subject | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS ID | WOS:000479057200036 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/162325 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Jing; Zhang, Quanbin |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,et al. In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2559-2571. |
| APA | Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,Wang, Jing,&Zhang, Quanbin.(2019).In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells.JOURNAL OF APPLIED PHYCOLOGY,31(4),2559-2571. |
| MLA | Liu, YingJuan,et al."In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2559-2571. |
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