In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells

doi:10.1007/s10811-018-1713-x

IOCAS-IR > 实验海洋生物学重点实验室

	In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells
	Liu, YingJuan 1,2,3; Deng, Zhenzhen 1,2,3; Geng, Lihua 1,2,3; Wang, Jing1,2 ; Zhang, Quanbin 1,2
	2019-08-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	31 期号:4 页码:2559-2571
通讯作者	Wang, Jing(jingwang@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
摘要	Porphyran, derived from the marine red algae Porphyra and Pyropia, is a type of polysaccharide. Oligo-porphyran (OP) was prepared from porphyran (isolated from Pyropia yezoensis) through the acidolysis reaction containing a linear backbone of alternating 3-linked beta-d-galactose and 4-linked alpha-l-galactose-6-sulfate. Our previous studies have shown that oligo-porphyran could attenuate MPTP-induced behavioral deficits in mice by inhibiting the tyrosine hydroxylase (TH) loss. In the present study, we further investigated how OP protects the dopamine neurons from loss in PC12 cells. OP significantly attenuated LDH release and the production of reactive oxygen species (ROS) lesioned by 6-OHDA. Moreover, OP alleviated the mitochondrial membrane potential (MMP) loss, the ratio of Bax/Bcl-2, and upregulated the PI3K/ Akt/PKC pathway, the level of tyrosine hydroxylase (TH), and dopamine transporter (DAT). Furthermore, OP decreased the production of proinflammatory cytokines. Terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining further indicated that OP treatment inhibited apoptosis. These results might contribute to the overall understanding of the potential health benefits of OP for food and drug applications.
关键词	Rhodophyta Oligo-porphyran Apoptosis 6-OHDA PC12 cells Mitochondria
DOI	10.1007/s10811-018-1713-x
收录类别	SCI
语种	英语
资助项目	National Key RD Plan[2018YFD0901104] ; Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of the Chinese Academy of Sciences[ZSTH-025] ; Natural Science Foundation of China[41406144] ; Natural Science Foundation of China[41506165] ; Ocean Public Welfare Scientific Research Project ; State Oceanic Administration of the People's Republic of China[201405040] ; Science and Technology Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] ; National Key RD Plan[2018YFD0901104] ; Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of the Chinese Academy of Sciences[ZSTH-025] ; Natural Science Foundation of China[41406144] ; Natural Science Foundation of China[41506165] ; Ocean Public Welfare Scientific Research Project ; State Oceanic Administration of the People's Republic of China[201405040] ; Science and Technology project of Shandong province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000479057200036
出版者	SPRINGER
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162325
专题	实验海洋生物学重点实验室
通讯作者	Wang, Jing; Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,et al. In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2559-2571.
APA	Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,Wang, Jing,&Zhang, Quanbin.(2019).In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells.JOURNAL OF APPLIED PHYCOLOGY,31(4),2559-2571.
MLA	Liu, YingJuan,et al."In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2559-2571.

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