IOCAS-IR  > 实验海洋生物学重点实验室
In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells
Liu, YingJuan1,2,3; Deng, Zhenzhen1,2,3; Geng, Lihua1,2,3; Wang, Jing1,2; Zhang, Quanbin1,2
2019-08-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume31Issue:4Pages:2559-2571
Corresponding AuthorWang, Jing(jingwang@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractPorphyran, derived from the marine red algae Porphyra and Pyropia, is a type of polysaccharide. Oligo-porphyran (OP) was prepared from porphyran (isolated from Pyropia yezoensis) through the acidolysis reaction containing a linear backbone of alternating 3-linked beta-d-galactose and 4-linked alpha-l-galactose-6-sulfate. Our previous studies have shown that oligo-porphyran could attenuate MPTP-induced behavioral deficits in mice by inhibiting the tyrosine hydroxylase (TH) loss. In the present study, we further investigated how OP protects the dopamine neurons from loss in PC12 cells. OP significantly attenuated LDH release and the production of reactive oxygen species (ROS) lesioned by 6-OHDA. Moreover, OP alleviated the mitochondrial membrane potential (MMP) loss, the ratio of Bax/Bcl-2, and upregulated the PI3K/ Akt/PKC pathway, the level of tyrosine hydroxylase (TH), and dopamine transporter (DAT). Furthermore, OP decreased the production of proinflammatory cytokines. Terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining further indicated that OP treatment inhibited apoptosis. These results might contribute to the overall understanding of the potential health benefits of OP for food and drug applications.
KeywordRhodophyta Oligo-porphyran Apoptosis 6-OHDA PC12 cells Mitochondria
DOI10.1007/s10811-018-1713-x
Indexed BySCI
Language英语
Funding ProjectNational Key RD Plan[2018YFD0901104] ; Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of the Chinese Academy of Sciences[ZSTH-025] ; Natural Science Foundation of China[41406144] ; Natural Science Foundation of China[41506165] ; Ocean Public Welfare Scientific Research Project ; State Oceanic Administration of the People's Republic of China[201405040] ; Science and Technology project of Shandong province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000479057200036
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162325
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Jing; Zhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,et al. In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2559-2571.
APA Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,Wang, Jing,&Zhang, Quanbin.(2019).In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells.JOURNAL OF APPLIED PHYCOLOGY,31(4),2559-2571.
MLA Liu, YingJuan,et al."In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2559-2571.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, YingJuan]'s Articles
[Deng, Zhenzhen]'s Articles
[Geng, Lihua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, YingJuan]'s Articles
[Deng, Zhenzhen]'s Articles
[Geng, Lihua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, YingJuan]'s Articles
[Deng, Zhenzhen]'s Articles
[Geng, Lihua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.