MITF Regulates Downstream Genes in Response to Vibrio parahaemolyticus Infection in the Clam Meretrix Petechialis

doi:10.3389/fimmu.2019.01547

IOCAS-IR > 实验海洋生物学重点实验室

	MITF Regulates Downstream Genes in Response to Vibrio parahaemolyticus Infection in the Clam Meretrix Petechialis
	Zhang, Shujing 1,2; Yue, Xin 1,3; Yu, Jiajia1,2 ; Wang, Hongxia 1,3; Liu, Baozhong1,2,3
	2019-07-04
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	10 页码:12
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
摘要	The microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix-leucine zipper protein that plays a key role in cell proliferation, survival and immune defense through the direct transcriptional control of downstream genes. We have found that MITF participates in the immune response to Vibrio parahaemolyticus infection in the clam Meretrix petechialis. In this study, we focused on how MITF functions in immunity. First, PO, CTSK, and BCL-2 were identified as the target genes of MpMITF in the clam by RNAi. EMSAs showed direct binding between the MpMITF protein and the E-box of the MpPO, MpCTSK, and MpBCL-2 promoters. Yeast one-hybrid assays also suggested that MpMITF could activate the expression of these three downstream genes. These results demonstrated that the transcriptional expression of MpPO, MpCTSK, and MpBCL-2 is directly regulated by MpMITF. Second, we analyzed the roles of MpPO, MpCTSK, and MpBCL-2 in clam immunity. The mRNA expression of MpPO, MpCTSK, and MpBCL-2 increased significantly after V. parahaemolyticus challenge, which implied that these genes might take part in the immune defense against V. parahaemolyticus challenge in clams. The purified recombinant proteins, MpPO and MpCTSK, inhibited the growth of V. parahaemolyticus. Additionally, the apoptosis rate of clam haemocytes rose significantly when the activity of MpBCL-2 was suppressed. These results revealed that MpPO, MpCTSK, and MpBCL-2 are involved in the immune defense against V. parahaemolyticus. This study supports the idea that the MpMITF pathway plays a key role in immune defense through the direct regulation of the downstream genes MpPO, MpCTSK, and MpBCL-2 in the clam, M. petechialis.
关键词	MITF signaling pathway Vibrio parahaemolyticus clam immune response
DOI	10.3389/fimmu.2019.01547
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; China Agriculture Research System[CARS-49] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology[YQ2018NO07] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; China Agriculture Research System[CARS-49] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology[YQ2018NO07]
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000474208300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162002
专题	实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Shujing,Yue, Xin,Yu, Jiajia,et al. MITF Regulates Downstream Genes in Response to Vibrio parahaemolyticus Infection in the Clam Meretrix Petechialis[J]. FRONTIERS IN IMMUNOLOGY,2019,10:12.
APA	Zhang, Shujing,Yue, Xin,Yu, Jiajia,Wang, Hongxia,&Liu, Baozhong.(2019).MITF Regulates Downstream Genes in Response to Vibrio parahaemolyticus Infection in the Clam Meretrix Petechialis.FRONTIERS IN IMMUNOLOGY,10,12.
MLA	Zhang, Shujing,et al."MITF Regulates Downstream Genes in Response to Vibrio parahaemolyticus Infection in the Clam Meretrix Petechialis".FRONTIERS IN IMMUNOLOGY 10(2019):12.

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