Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China

doi:10.1002/jobm.201800674

IOCAS-IR

	Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China
	Sun, Wuyang 1; Ali, Imran 1; Liu, Jiwei 1; Dai, Min 2; Cao, Wenrui 3; Jiang, Mingyu 3; Saren, Gaowa 3; Yu, Xinke 3; Peng, Changsheng 1,2; Naz, Iffat 4,5
	2019-07-01
发表期刊	JOURNAL OF BASIC MICROBIOLOGY
ISSN	0233-111X
卷号	59 期号:7 页码:723-734
通讯作者	Peng, Changsheng(cspeng@ouc.edu.cn) ; Naz, Iffat(iffatkhattak@yahoo.com)
摘要	In the present study, 12 indigenous diesel-oil-degrading bacteria were isolated from the petroleum-contaminated soils of the Changqing oil field (Xi'an, China). Measurement of the diesel-oil degradation rates of these strains by the gravimetric method revealed that they ranged from 42% to 66% within 2 weeks. The highest degradation rates were observed from strains CQ8-1 (66%), CQ8-2 (62.6%), and CQ11 (59%), which were identified as Bacillus thuringiensis, Ochrobactrum anthropi, and Bordetella bronchialis, respectively, based on their 16S rDNA sequences. Moreover, the physiological and biochemical properties of these three strains were analyzed by Gram staining, catalase, oxidase, and Voges-Proskauer tests. Transmission electron microscopy showed that all three strains were rod shaped with flagella. Gas chromatography and mass spectrometric analyses indicated that medium- and long-chain n-alkanes in diesel oil (C11-C29) were degraded to different degrees by B. thuringiensis, O. anthropi, and B. bronchialis, and the degradation rates gradually decreased as the carbon numbers increased. Overall, the results of this study indicate strains CQ8-1, CQ8-2, and CQ11 might be useful for environmentally friendly and cost-effective bioremediation of oil-contaminated soils.
关键词	Bacillus thuringiensis Bordetella bronchialis diesel-oil biodegradation Ochrobactrum anthropi
DOI	10.1002/jobm.201800674
收录类别	SCI
语种	英语
资助项目	Shandong Province Key Research and Development Program[2016GSF115040] ; State Key Laboratory of Environmental Criteria and Risk Assessment[SKLECRA2013FP12] ; Shandong Province Key Research and Development Program[2016GSF115040] ; State Key Laboratory of Environmental Criteria and Risk Assessment[SKLECRA2013FP12]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000474283500006
出版者	WILEY
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161999
专题	中国科学院海洋研究所
通讯作者	Peng, Changsheng; Naz, Iffat
作者单位	1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Minist Educ, Qingdao, Shandong, Peoples R China 2.Zhaoqing Univ, Sch Environm & Chem Engn, Zhaoqing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China 4.Qassim Univ, Dept Biol, Buraydah 51452, Saudi Arabia 5.Quaid I Azam Univ, Dept Microbiol, Islamabad, Pakistan
推荐引用方式 GB/T 7714	Sun, Wuyang,Ali, Imran,Liu, Jiwei,et al. Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China[J]. JOURNAL OF BASIC MICROBIOLOGY,2019,59(7):723-734.
APA	Sun, Wuyang.,Ali, Imran.,Liu, Jiwei.,Dai, Min.,Cao, Wenrui.,...&Naz, Iffat.(2019).Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China.JOURNAL OF BASIC MICROBIOLOGY,59(7),723-734.
MLA	Sun, Wuyang,et al."Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China".JOURNAL OF BASIC MICROBIOLOGY 59.7(2019):723-734.

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