IOCAS-IR  > 实验海洋生物学重点实验室
A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts
Fan, Bolin1; Chen, Funuan1; Li, Yuan1; Wang, Zhongliang1; Wang, Zhiwen1; Lu, Yishan1,2; Wu, Zaohe1; Jian, Jichang1,3,4; Wang, Bei1,3,4
2019-06-01
Source PublicationMOLECULAR OMICS
Volume15Issue:3Pages:233-246
Corresponding AuthorWang, Bei(wong19820204@126.com)
AbstractThe pathogenesis of tilapia meningoencephalitis is still unclear, where the involvement of circRNA is considered for its active role as a `` miRNA sponge''. Therefore, we aimed to investigate the profile of circRNA in tilapia meningoencephalitis further by constructing the circRNA-miRNA network for in-depth mechanism exploration. Briefly, a nile tilapia model of meningitis was established by injecting Streptococcus agalactiae (1.0 x 10(7) cfu per mL) and we evaluated the infected tilapia brain for the expression profile of circRNAs, their potential functions and their correlation with genes involved in inflammatory pathways. A total of 11 263 circRNAs were identified from RNA sequencing (RNA-seq) data in nile tilapia (Oreochromis niloticus), a commercially important fish in China and East Asia. GO and KEGG analyses revealed that the biological functions of genes hosting the circRNAs were enriched in the progression of metabolism and binding. Notably, we found that 99% circRNAs in tilapia had abundant miRNA-binding sites and a total of 2136 of the identified circRNAs had two to six miRNA-binding sites. Six circRNAs were validated by qRT-PCR and the final circRNA-miRNA network was constructed. This is the first report of comprehensive identification of O. niloticus circRNAs being differentially regulated in the brain in normal conditions relating to S. agalactiae infection. This work will shed novel light on gene expression mechanisms under disease conditions and may identify circRNAs as new biomarkers for meningoencephalitis and neurodegenerative disorders.
DOI10.1039/c9mo00025a
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702386] ; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000474156900004
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161930
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Bei
Affiliation1.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim Guangdong Higher E, Coll Fishery, Zhanjiang, GD, Peoples R China
2.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, GD, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fan, Bolin,Chen, Funuan,Li, Yuan,et al. A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts[J]. MOLECULAR OMICS,2019,15(3):233-246.
APA Fan, Bolin.,Chen, Funuan.,Li, Yuan.,Wang, Zhongliang.,Wang, Zhiwen.,...&Wang, Bei.(2019).A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts.MOLECULAR OMICS,15(3),233-246.
MLA Fan, Bolin,et al."A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts".MOLECULAR OMICS 15.3(2019):233-246.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fan, Bolin]'s Articles
[Chen, Funuan]'s Articles
[Li, Yuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fan, Bolin]'s Articles
[Chen, Funuan]'s Articles
[Li, Yuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fan, Bolin]'s Articles
[Chen, Funuan]'s Articles
[Li, Yuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.