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A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts
Fan, Bolin1; Chen, Funuan1; Li, Yuan1; Wang, Zhongliang1; Wang, Zhiwen1; Lu, Yishan1,2; Wu, Zaohe1; Jian, Jichang1,3,4; Wang, Bei1,3,4
2019-06-01
发表期刊MOLECULAR OMICS
卷号15期号:3页码:233-246
通讯作者Wang, Bei(wong19820204@126.com)
摘要The pathogenesis of tilapia meningoencephalitis is still unclear, where the involvement of circRNA is considered for its active role as a `` miRNA sponge''. Therefore, we aimed to investigate the profile of circRNA in tilapia meningoencephalitis further by constructing the circRNA-miRNA network for in-depth mechanism exploration. Briefly, a nile tilapia model of meningitis was established by injecting Streptococcus agalactiae (1.0 x 10(7) cfu per mL) and we evaluated the infected tilapia brain for the expression profile of circRNAs, their potential functions and their correlation with genes involved in inflammatory pathways. A total of 11 263 circRNAs were identified from RNA sequencing (RNA-seq) data in nile tilapia (Oreochromis niloticus), a commercially important fish in China and East Asia. GO and KEGG analyses revealed that the biological functions of genes hosting the circRNAs were enriched in the progression of metabolism and binding. Notably, we found that 99% circRNAs in tilapia had abundant miRNA-binding sites and a total of 2136 of the identified circRNAs had two to six miRNA-binding sites. Six circRNAs were validated by qRT-PCR and the final circRNA-miRNA network was constructed. This is the first report of comprehensive identification of O. niloticus circRNAs being differentially regulated in the brain in normal conditions relating to S. agalactiae infection. This work will shed novel light on gene expression mechanisms under disease conditions and may identify circRNAs as new biomarkers for meningoencephalitis and neurodegenerative disorders.
DOI10.1039/c9mo00025a
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31702386] ; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037] ; National Natural Science Foundation of China[31702386] ; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037]
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000474156900004
出版者ROYAL SOC CHEMISTRY
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/161930
专题实验海洋生物学重点实验室
通讯作者Wang, Bei
作者单位1.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim Guangdong Higher E, Coll Fishery, Zhanjiang, GD, Peoples R China
2.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, GD, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
通讯作者单位中国科学院海洋研究所
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Fan, Bolin,Chen, Funuan,Li, Yuan,et al. A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts[J]. MOLECULAR OMICS,2019,15(3):233-246.
APA Fan, Bolin.,Chen, Funuan.,Li, Yuan.,Wang, Zhongliang.,Wang, Zhiwen.,...&Wang, Bei.(2019).A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts.MOLECULAR OMICS,15(3),233-246.
MLA Fan, Bolin,et al."A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts".MOLECULAR OMICS 15.3(2019):233-246.
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