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Hemolymph C1qDC promotes the phagocytosis of oyster Crassostrea gigas hemocytes by interacting with the membrane receptor beta-integrin
Lv, Zhao1,2,3; Wang, Lingling2,4,5; Jia, Zhihao1,3; Sun, Jiejie4,5; Wang, Weilin4,5; Liu, Zhaoqun4,5; Qiu, Limei1; Wang, Mengqiang1; Song, Linsheng2,4,5
2019-09-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume98Pages:42-53
Corresponding AuthorSong, Linsheng(lshsong@dlou.edu.cn)
AbstractPhagocytosis constitutes a conserved cellular process for multicellular animals to ingest or engulf other cells or particles, which is facilitated by the use of opsonins to bind foreign particles and interact with cell surface receptors. The invertebrate secreted CIA domain-containing proteins (C1qDCs) have been reported to exhibit opsonic activity, while the detailed mechanisms of opsonization still remain unclear. In the present study, a C1qDC (designated as CgC1qDC-5) with opsonic activity was identified from the hemolymph of oyster Crassostrea gigas. CgC1qDC-5 exhibited the ability to bind pathogen-associated molecular patterns (PAMPs) of lipopolysaccharides (LPS) and Lipid A. It could also bind and agglutinate Gram-negative bacteria Escherichia coli, Vibrio splendidus and Vibrio anguillarum, whereas the agglutinating activity could be inhibited by LPS. In addition, CgC1qDC-5 could enhance the phagocytosis of hemocytes toward E. coli, V. splendidus, and V. anguillarum. GST pull-down and surface plasmon resonance assays in vitro revealed that CgC1qDC-5 could interact with beta-integrin (CgIntegrin). In vivo, CgC1qDC-5 was observed to bind hemocytes and co-localized with CgIntegrin on the cell membrane of hemocytes. Antibody-mediated blockage of CgIntegrin hindered the CgC1qDC-5-enhanced hemocytic phagocytosis. CgIntegrin also exhibited the ability to bind the Gram-negative bacteria E. coli, V. splendidus, V. anguillarum and Vibrio parahaemolyticus, and PAMP of LPS, but not Lipid A. A phagocytosis assay demonstrated that CgIntegrin could directly mediate phagocytosis toward bacteria as a phagocytic receptor. These results collectively suggested that CgC1qDC-5 could serve as an opsonin to recognize and bind bacteria, and subsequently interact with CgIntegrin on the hemocyte surface to enhance the CgIntegrin-mediated phagocytosis in oyster.
KeywordPhagocytosis C1qDC Opsonization beta-Integrin Oyster Crassostrea gigas
DOI10.1016/j.dci.2019.04.004
Indexed BySCI
Language英语
Funding ProjectNational Key RD Projects[2018YFD0900502] ; National Science Foundation of China[U1706204] ; AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Key R&D Program of Liaoning Province[201703165] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research ; earmarked fund from Modern Agro-industry Technology Research System[CARS-48] ; Distinguished Professor of Liaoning, Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000472705700006
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161802
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
5.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lv, Zhao,Wang, Lingling,Jia, Zhihao,et al. Hemolymph C1qDC promotes the phagocytosis of oyster Crassostrea gigas hemocytes by interacting with the membrane receptor beta-integrin[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,98:42-53.
APA Lv, Zhao.,Wang, Lingling.,Jia, Zhihao.,Sun, Jiejie.,Wang, Weilin.,...&Song, Linsheng.(2019).Hemolymph C1qDC promotes the phagocytosis of oyster Crassostrea gigas hemocytes by interacting with the membrane receptor beta-integrin.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,98,42-53.
MLA Lv, Zhao,et al."Hemolymph C1qDC promotes the phagocytosis of oyster Crassostrea gigas hemocytes by interacting with the membrane receptor beta-integrin".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 98(2019):42-53.
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