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The interrelation between photorespiration and astaxanthin accumulation in Haematococcus pluvialis using metabolomic analysis
Zhang, Chunhui1,2,3; Zhang, Litao1,2,4; Liu, Jianguo1,2,4
2019-08-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume41Pages:10
Corresponding AuthorLiu, Jianguo(jgliu@qdio.ac.cn)
AbstractTo further investigate the interrelation between photorespiration and astaxanthin accumulation, metabolomic analysis of Haematococcus pluvialis during the astaxanthin accumulation period was performed, using gas chromatography-mass spectrometry (GC-MS), in the absence or presence of carboxymethoxylamine (CM), an inhibitor of the photorespiratory pathway. Algal samples were collected on days 0, 6 and 12, which were named as the green phase with green, non-motile cells, the brown phase with astaxanthin-synthesizing, non-motile cells, and the red phase with astaxanthin-enriched, red, non-motile cells, respectively. The inhibition of photo-respiration by CM did not affect the biomass, had a slight effect on chlorophyll at the end of the incubation, but significantly suppressed astaxanthin accumulation. A total of 78 metabolites were identified by GC-MS, including 27 amino acids, 16 organic acids, 12 fatty acids, 7 polyols, 3 phosphoric acids, 3 sugars, 2 amines, and 8 other compounds. Multivariate statistical analyses (principal component analysis, partial least squares-discriminant analysis), and hierarchical cluster analysis revealed the clustering of the metabolites. Glycine and glycolic acid had accumulated substantially at both the brown and red phases, indicating that photorespiration was inhibited by CM. In presence of CM, the TCA cycle was restricted at the brown phase due to decreased intermediates, specifically, decreased levels of fructose and glucose. However, inhibiting photorespiration enhanced the levels of many intracellular cytoprotective metabolites, such as amino acids, polyamines, polyols and sucrose. A hypothetical metabolic regulation model of the photorespiratory pathway affecting astaxanthin accumulation of H. pluvialis is proposed. This study provides the first metabolomic evidence that photorespiration enhances astaxanthin accumulation.
KeywordAstaxanthin accumulation Haematococcus pluvialis Metabolomic analysis Photorespiration
DOI10.1016/j.algal.2019.101520
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572639] ; National Natural Science Foundation of China[U1706209] ; Suzhou, China
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000472593800055
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161796
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Jianguo
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Yunnan Alphy Biotech Co Ltd, Natl Local Joint Engn Res Ctr Haematococcus Pluvi, Chuxiong 675012, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Chunhui,Zhang, Litao,Liu, Jianguo. The interrelation between photorespiration and astaxanthin accumulation in Haematococcus pluvialis using metabolomic analysis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,41:10.
APA Zhang, Chunhui,Zhang, Litao,&Liu, Jianguo.(2019).The interrelation between photorespiration and astaxanthin accumulation in Haematococcus pluvialis using metabolomic analysis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,41,10.
MLA Zhang, Chunhui,et al."The interrelation between photorespiration and astaxanthin accumulation in Haematococcus pluvialis using metabolomic analysis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 41(2019):10.
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