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Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein
Wang, Xueqin1,2; Yu, Huahua1,2; Xing, Ronge1,2; Liu, Song1,2; Chen, Xiaolin1,2; Li, Pengcheng1,2
2019-05-02
Source PublicationMOLECULES
ISSN1420-3049
Volume24Issue:10Pages:17
Corresponding AuthorLi, Pengcheng(pcli@qdio.ac.cn)
AbstractThe aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 +/- 0.05 mg/mL and 1.04 +/- 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification.
Keywordpacific herring protein hydrolysate peptides antioxidant activity purification
DOI10.3390/molecules24101946
Indexed BySCI
Language英语
Funding ProjectKey Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006] ; Qingdao Science and Technology Project[17-3-3-21-nsh]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000470996600104
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161784
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Pengcheng
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein[J]. MOLECULES,2019,24(10):17.
APA Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2019).Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein.MOLECULES,24(10),17.
MLA Wang, Xueqin,et al."Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein".MOLECULES 24.10(2019):17.
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