Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein

doi:10.3390/molecules24101946

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	Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein
	Wang, Xueqin 1,2; Yu, Huahua1,2 ; Xing, Ronge1,2 ; Liu, Song 1,2; Chen, Xiaolin 1,2; Li, Pengcheng1,2
	2019-05-02
Source Publication	MOLECULES
ISSN	1420-3049
Volume	24 Issue:10 Pages:17
Corresponding Author	Li, Pengcheng(pcli@qdio.ac.cn)
Abstract	The aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 +/- 0.05 mg/mL and 1.04 +/- 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification.
Keyword	pacific herring protein hydrolysate peptides antioxidant activity purification
DOI	10.3390/molecules24101946
Indexed By	SCI
Language	英语
Funding Project	Key Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006] ; Qingdao Science and Technology Project[17-3-3-21-nsh]
WOS Research Area	Biochemistry & Molecular Biology ; Chemistry
WOS Subject	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS ID	WOS:000470996600104
Publisher	MDPI
Citation statistics	Cited Times:1[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.qdio.ac.cn/handle/337002/161784
Collection	实验海洋生物学重点实验室
Corresponding Author	Li, Pengcheng
Affiliation	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
First Author Affilication	Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author Affilication	Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation GB/T 7714	Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein[J]. MOLECULES,2019,24(10):17.
APA	Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2019).Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein.MOLECULES,24(10),17.
MLA	Wang, Xueqin,et al."Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein".MOLECULES 24.10(2019):17.

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