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Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein
Wang, Xueqin1,2; Yu, Huahua1,2; Xing, Ronge1,2; Liu, Song1,2; Chen, Xiaolin1,2; Li, Pengcheng1,2
2019-05-02
发表期刊MOLECULES
ISSN1420-3049
卷号24期号:10页码:17
通讯作者Li, Pengcheng(pcli@qdio.ac.cn)
摘要The aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 +/- 0.05 mg/mL and 1.04 +/- 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification.
关键词pacific herring protein hydrolysate peptides antioxidant activity purification
DOI10.3390/molecules24101946
收录类别SCI
语种英语
资助项目Key Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006] ; Qingdao Science and Technology Project[17-3-3-21-nsh] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006] ; Qingdao Science and Technology Project[17-3-3-21-nsh]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号WOS:000470996600104
出版者MDPI
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/161784
专题实验海洋生物学重点实验室
通讯作者Li, Pengcheng
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein[J]. MOLECULES,2019,24(10):17.
APA Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2019).Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein.MOLECULES,24(10),17.
MLA Wang, Xueqin,et al."Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein".MOLECULES 24.10(2019):17.
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