Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein | |
Wang, Xueqin1,2; Yu, Huahua1,2 ; Xing, Ronge1,2; Liu, Song1,2; Chen, Xiaolin1,2; Li, Pengcheng1,2
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| 2019-05-02 | |
| Source Publication | MOLECULES
 |
| ISSN | 1420-3049 |
| Volume | 24Issue:10Pages:17 |
| Corresponding Author | Li, Pengcheng(pcli@qdio.ac.cn) |
| Abstract | The aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 +/- 0.05 mg/mL and 1.04 +/- 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification. |
| Keyword | pacific herring protein hydrolysate peptides antioxidant activity purification |
| DOI | 10.3390/molecules24101946 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Key Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006] ; Qingdao Science and Technology Project[17-3-3-21-nsh] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS ID | WOS:000470996600104 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/161784 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Li, Pengcheng |
| Affiliation | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein[J]. MOLECULES,2019,24(10):17. |
| APA | Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2019).Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein.MOLECULES,24(10),17. |
| MLA | Wang, Xueqin,et al."Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein".MOLECULES 24.10(2019):17. |
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