Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae | |
| Wu, Lele1,2,3,4; Han, Mingming1,2,3; Song, Zongcheng5; Xu, Shihong1,2,3; Li, Jun1,2,3; Li, Xueqing1; Wang, Yanfeng1,2,3; Yue, Xinlu5; Li, Xian1,2,3 | |
| 2019-07-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 90页码:328-337 |
| 通讯作者 | Li, Jun(junli@qclio.ac.cn) ; Li, Xueqing(lixian@qdio.ac.cn) |
| 摘要 | Light is a key environmental factor that synchronizes various life stages from embryo development to sexual maturation in fish. For turbot, light spectra have the most influence at the larval and juvenile stages. In the current study, differences in the development of embryos and the performance of newly hatched turbot larvae exposed to five different spectra: full spectrum (LDF), blue (LOB, peak at 450 nm), green (LDG, peak at 533 nm), orange (LDO, peak at 595 nm) and red (LDR, peak at 629 nm), were examined. At 62.8 h post fertilization, a higher number of embryos exposed to short-wavelengths (LDG and LOB) had developed a heartbeat in comparison with embryos exposed to other wavelengths. Larvae exposed to the green spectrum had higher malformation rates than larvae exposed to the other spectra, indicating that larvae exposed to green light may have significantly reduced survival rates. The results of non-specific immunity parameters showed that the mRNA expression levels of cathepsin D (CTSD), cathepsin F (CTSF), catalase (CAT) and metallothionein (MT) in larvae exposed to LDB were significantly higher than those exposed to other spectra, but CAT activity in larvae exposed to LDB was significantly lower than larvae exposed to the other spectra. There was no significant difference in MT activity in larvae exposed to the five different spectra. The mRNA expression level of lysozyme (LZM) in larvae exposed to LDR was significantly higher than other spectra, while there was no significant difference in LZM activity observed in larvae exposed to LDR, LDG, LDB and LDF. The difference of the enzyme activity of total superoxide dismutase (T-SOD) was not significant among larvae exposed to the five spectra. mRNA expression of the heat shock protein 70 (HSP70) was significantly higher in newly hatched larvae exposed to LDB, LDR and LDG, indicating that larvae exposed to LDB, LDG and LDR exhibited a stress response. The mRNA expression level of the insulin-like growth factor-1 (IGF-1) and growth parameters in the newly hatched larvae exposed to the different spectra were not significantly different. The results of the present study indicate that LDO and LDF should be used for embryo incubation and newly hatched larvae when rearing turbot. This study provides a theoretical basis for optimizing the incubation light environment for fertilized turbot eggs, promoting immunity and reducing stress responses in newly hatched larvae. |
| 关键词 | Scophthalmus maximus Light spectrum Embryo Newly hatched larvae |
| DOI | 10.1016/j.fsi.2019.05.007 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key R&D Program of China[2017YFB0404000] ; Key R&D Program of Shandong Province[2018GHY115031] ; Scientific and Technologic Development Program of Weihai[2017GNS05] ; China Agriculture Research System[CARS-47] ; National Key R&D Program of China[2017YFB0404000] ; Key R&D Program of Shandong Province[2018GHY115031] ; Scientific and Technologic Development Program of Weihai[2017GNS05] ; China Agriculture Research System[CARS-47] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000471085000036 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/161781 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Jun; Li, Xueqing |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 通讯作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wu, Lele,Han, Mingming,Song, Zongcheng,et al. Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae[J]. FISH & SHELLFISH IMMUNOLOGY,2019,90:328-337. |
| APA | Wu, Lele.,Han, Mingming.,Song, Zongcheng.,Xu, Shihong.,Li, Jun.,...&Li, Xian.(2019).Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae.FISH & SHELLFISH IMMUNOLOGY,90,328-337. |
| MLA | Wu, Lele,et al."Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae".FISH & SHELLFISH IMMUNOLOGY 90(2019):328-337. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 1-s2.0-S105046481930（807KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
| Effects of different（807KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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