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The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica
Luan, Hexiang1,2,3; Yao, Jianting1,2,3; Chen, Zhihang1,2,3,4; Duan, Delin1,2,3
2019-05-02
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume20Issue:10Pages:12
Corresponding AuthorDuan, Delin(dlduan@qdio.ac.cn)
AbstractBlue light (BL) plays an important role in regulation of the growth and development of aquatic plants and land plants. Aureochrome (AUREO), the recent BL photoreceptor identified in photosynthetic stramenopile algae, is involved in the photomorphogenesis and early development of Saccharina japonica porophytes (kelp). However the factors that interact with the SjAUREO under BL conditions specifically are not clear. Here in our study, three high quality cDNA libraries with CFU over 5 x 10(6) and a recombination rate of 100% were constructed respectively through white light (WL), BL and darkness (DK) treatments to the juvenile sporophytes. Based on the constructed cDNA libraries, the interactors of SjAUREO were screened and analyzed. There are eighty-four genes encoding the sixteen predicted proteins from the BL cDNA library, sixty-eight genes encoding eighteen predicted proteins from the DK cDNA library, and seventy-four genes encoding nineteen proteins from the WL cDNA library. All the predicted proteins are presumed to interact with SjAUREO when co-expressed with SjAUREO seperately. The 40S ribosomal protein S6 (RPS6), which only exists in the BL treated cDNA library except for two other libraries, and which is essential for cell proliferation and is involved in cell cycle progression, was selected for detailed analysis. We showed that its transcription was up-regulated by BL, and was highly transcribed in the basal blade (meristem region) of juvenile sporophytes but less in the distal part. Taken together, our results indicated that RPS6 was highly involved in BL-mediated kelp cellular division and photomorphogenesis by interacting with SjAUREO.
KeywordSaccharina japonica aureochrome blue light yeast two hybrid 40S ribosomal protein S6
DOI10.3390/ijms20102414
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2017M612362] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; CAS-Fujian STS project
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000471001400045
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161775
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan, Delin
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Luan, Hexiang,Yao, Jianting,Chen, Zhihang,et al. The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(10):12.
APA Luan, Hexiang,Yao, Jianting,Chen, Zhihang,&Duan, Delin.(2019).The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(10),12.
MLA Luan, Hexiang,et al."The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.10(2019):12.
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