The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica

doi:10.3390/ijms20102414

IOCAS-IR > 实验海洋生物学重点实验室

	The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica
	Luan, Hexiang 1,2,3; Yao, Jianting 1,2,3; Chen, Zhihang 1,2,3,4; Duan, Delin 1,2,3
	2019-05-02
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	20 期号:10 页码:12
通讯作者	Duan, Delin(dlduan@qdio.ac.cn)
摘要	Blue light (BL) plays an important role in regulation of the growth and development of aquatic plants and land plants. Aureochrome (AUREO), the recent BL photoreceptor identified in photosynthetic stramenopile algae, is involved in the photomorphogenesis and early development of Saccharina japonica porophytes (kelp). However the factors that interact with the SjAUREO under BL conditions specifically are not clear. Here in our study, three high quality cDNA libraries with CFU over 5 x 10(6) and a recombination rate of 100% were constructed respectively through white light (WL), BL and darkness (DK) treatments to the juvenile sporophytes. Based on the constructed cDNA libraries, the interactors of SjAUREO were screened and analyzed. There are eighty-four genes encoding the sixteen predicted proteins from the BL cDNA library, sixty-eight genes encoding eighteen predicted proteins from the DK cDNA library, and seventy-four genes encoding nineteen proteins from the WL cDNA library. All the predicted proteins are presumed to interact with SjAUREO when co-expressed with SjAUREO seperately. The 40S ribosomal protein S6 (RPS6), which only exists in the BL treated cDNA library except for two other libraries, and which is essential for cell proliferation and is involved in cell cycle progression, was selected for detailed analysis. We showed that its transcription was up-regulated by BL, and was highly transcribed in the basal blade (meristem region) of juvenile sporophytes but less in the distal part. Taken together, our results indicated that RPS6 was highly involved in BL-mediated kelp cellular division and photomorphogenesis by interacting with SjAUREO.
关键词	Saccharina japonica aureochrome blue light yeast two hybrid 40S ribosomal protein S6
DOI	10.3390/ijms20102414
收录类别	SCI
语种	英语
资助项目	China Postdoctoral Science Foundation[2017M612362] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; CAS-Fujian STS project ; China Postdoctoral Science Foundation[2017M612362] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; CAS-Fujian STS project
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000471001400045
出版者	MDPI
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161775
专题	实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Luan, Hexiang,Yao, Jianting,Chen, Zhihang,et al. The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(10):12.
APA	Luan, Hexiang,Yao, Jianting,Chen, Zhihang,&Duan, Delin.(2019).The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(10),12.
MLA	Luan, Hexiang,et al."The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.10(2019):12.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
The 40S Ribosomal Pr（1921KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览