IOCAS-IR  > 实验海洋生物学重点实验室
Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides
Ning, Junhao1,3,4; Liu, Yuan1,2,3; Gao, Fengtao1,2,3; Song, Chengwen1,2,3; Cui, Zhaoxia2,5
2019-06-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号89页码:574-585
通讯作者Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
摘要Alpha-2 macroglobulin (A2M) is a ubiquitous protease inhibitor involved in the innate host defense system. Herein, two distinct A2M genes (designated as PtA2M-1 and PtA2M-2, respectively) were isolated from the swimming crab Portunus trituberculatus. PtA2M-1 and PtA2M-2 encoded proteins with 1541 or 1516 amino acids, respectively, containing the typically functional domains of A2M. Unlike highly expressed in hemocytes of most arthropods, PtA2M-1 and PtA2M-2 were predominantly detected in gill, eyestalk and digestive tracks. During the embryonic stages, PtA2Ms were found to be expressed most highly in fertilized eggs, suggesting their maternal origin. After challenged with Vibrio alginolyticus, the transcripts of PtA2Ms showed similar time-dependent response expression pattern, while PtA2M-1 was more sensitive to Micrococcus luteus and Pichia pastoris infection than PtA2M-2. Knockdown of PtA2M-1 or PtA2M-2 could significantly enhance the expression of propheno-loxidase (proPO) associated genes (PtproPO and PtPPAF) and serine protease related genes (PtcSP1-3 and PtSPH), however, PtLSZ and the phagocytosis-related genes (PtMyosin and PtRab5) were effectively inhibited. These results were further supported by the PO and lysozyme activities in hemolymph of the PtA2M-1- or PtA2M-2-silenced crabs. In addition, PtA2M-1 and PtA2M-2 could regulate the expression of antimicrobial peptide (AMP) genes (PtALF1-3, PtCrustin1 and PtCrustin3) through the Toll and NE-kappa B pathways. Our findings together suggest that PtA2Ms might function in crab host defense via regulating the proPO system, phagocytosis and the expression of AMP genes.
关键词Portunus trituberculatus Alpha-2 macroglobulin Expression profiles Phagocytosis Prophenoloxidase system AMP
DOI10.1016/j.fsi.2019.04.033
收录类别SCI
语种英语
资助项目National Key R&D Program of China[2018YFD0900303] ; National Natural Science Foundation of China of China[41776159] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001] ; National Key R&D Program of China[2018YFD0900303] ; National Natural Science Foundation of China of China[41776159] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000469897700064
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/161653
专题实验海洋生物学重点实验室
通讯作者Cui, Zhaoxia
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Ning, Junhao,Liu, Yuan,Gao, Fengtao,et al. Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:574-585.
APA Ning, Junhao,Liu, Yuan,Gao, Fengtao,Song, Chengwen,&Cui, Zhaoxia.(2019).Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides.FISH & SHELLFISH IMMUNOLOGY,89,574-585.
MLA Ning, Junhao,et al."Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides".FISH & SHELLFISH IMMUNOLOGY 89(2019):574-585.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Two alpha-2 macroglo(3369KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ning, Junhao]的文章
[Liu, Yuan]的文章
[Gao, Fengtao]的文章
百度学术
百度学术中相似的文章
[Ning, Junhao]的文章
[Liu, Yuan]的文章
[Gao, Fengtao]的文章
必应学术
必应学术中相似的文章
[Ning, Junhao]的文章
[Liu, Yuan]的文章
[Gao, Fengtao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。