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Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus
Zhang, Hongxia1,2,3; Wang, Qing3,4; Liu, Shilin1,2,3; Huo, Da1,2,3,5; Zhao, Jianmin3,4; Zhang, Libin1,2,3,5; Zhao, Ye6; Sun, Lina1,2,3,5; Yang, Hongsheng1,2,3,5
2019-06-04
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume10Pages:11
Corresponding AuthorSun, Lina(sunlina@qdio.ac.cn) ; Yang, Hongsheng(hshyang@qdio.ac.cn)
AbstractHost-intestine microbiota interactions have been widely studied in aquatic animals, but these interactions in the intestine regeneration process of the sea cucumber Apostichopus japonicus have been rarely investigated. To understand how intestine regeneration impacts the developing intestinal microbiome composition and function, we performed a case study to characterize the intestinal microbial composition and functional genes of A. japonicus during intestine regeneration stages. High-throughput 16S rRNA gene sequencing revealed significantly different intestine microbiota compositions in different regeneration stages. The phylogenetic diversity and composition of the intestinal microbiota changed significantly in the early regeneration stage and tended to recover in the end stage. During the regeneration process, the abundance of Bacteroidetes and Rhodobacterales increased significantly. A network analysis revealed that Rhodobacteraceae and Flavobacteriaceae may function as keystone taxa in the intestinal microbial community of A. japonicus during intestine regeneration. Metagenomic analyses of representative samples revealed that the microbiomes of regenerating intestines were enriched in genes facilitating cell proliferation, digestion and immunity. The increased abundance of Bacteroidetes elevated the enrichment of genes associated with carbohydrate utilization. Some functional features in the subsystem category changed in a pattern that was consistent with the changing pattern of microbiota composition during intestine regeneration. Our results revealed that seemingly regular alterations in the intestinal microbiome composition and function are associated with intestine regeneration stages. Intestinal microbiota can increase the abundance of beneficial bacterial members and upregulate related functional genes to adapt to intestine regeneration and reconstruct a stable community structure. This study provides a new insight into the mechanism of the host-microbiota interaction response to organ regeneration.
KeywordApostichopus japonicus intestine regeneration intestinal microbiota genomic metagenomic
DOI10.3389/fmicb.2019.01165
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41776162] ; China Postdoctoral Science Foundation[2018M642712] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science ; Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-09] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; Taishan Scholars Program ; Key Research Program of the Chinese Academy of Sciences[KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai[Y839081021] ; Youth Innovation Promotion Association CAS[2016196] ; Youth Innovation Promotion Association CAS[2019209]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000470133300001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161638
Collection海洋生态与环境科学重点实验室
Corresponding AuthorSun, Lina; Yang, Hongsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Yantai Univ, Ocean Sch, Yantai, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Hongxia,Wang, Qing,Liu, Shilin,et al. Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN MICROBIOLOGY,2019,10:11.
APA Zhang, Hongxia.,Wang, Qing.,Liu, Shilin.,Huo, Da.,Zhao, Jianmin.,...&Yang, Hongsheng.(2019).Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus.FRONTIERS IN MICROBIOLOGY,10,11.
MLA Zhang, Hongxia,et al."Genomic and Metagenomic Insights Into the Microbial Community in the Regenerating Intestine of the Sea Cucumber Apostichopus japonicus".FRONTIERS IN MICROBIOLOGY 10(2019):11.
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