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Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada
Zhang Wenjing1,2; Jin Weihua1,3; Duan Delin1,4,5,6; Zhang Quanbin1,4,6
2019-05-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume37Issue:3Pages:806-814
Corresponding AuthorDuan Delin(dlduan@qdio.ac.cn) ; Zhang Quanbin(qbzhang@qdio.ac.cn)
AbstractPolysaccharides were extracted from Grateloupia livida (Harv.) Yamada using hot water (extracted product denoted WGW) and then degraded in dilute sulfuric acid (degraded product denoted WGWD). The degraded mixture was then separated into four fractions through anion exchange chromatography on 2-diethylaminoethanol (DEAE)-Bio-Gel Agarose FF gel. Electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) was performed to elucidate the structural features of all fractions. In combination with nuclear magnetic resonance spectroscopy and infrared spectroscopy (IR) data, the major polysaccharide structures were concluded to be mu-carrageenan and kappa-carrageenan. mu-Carrageenan usually has a backbone of alternating 1,3-linked beta-D-galactopyranose residues sulfated at C-4 and 1,4-linked alpha-D-galactopyranose residues sulfated at C-6, while kappa-carrageenan consists of alternating 1,3-linked beta-D-galactopyranose residues sulfated at C-4 and 1,4-linked alpha-D-3,6-anhydrogalactopyranose residues. Trace nu-carrageenan, composed of 1,3-linked beta-D-galactopyranose residues sulfated at C-4 and 1,4-linked alpha-D-galactopyranose residues sulfated at C-2 and C-6, was also detected. Furthermore, the polysaccharide had a backbone comprising 1,3-linked beta-D-galactopyranose and 1,4-linked alpha-L-galactopyranose sulfated at C-6, which is the agarose precursor. The hydroxy groups in the galactopyranose were partially substituted by methyl and pyruvic acid acetal (PA) groups. The anticomplementary activities of WGW and its derivatives against classical pathways were measured. The native polysaccharides in WGW had higher activities, while the derivatives had much weaker activities. This indicated that the molecular weight and sulfate content were important factors affecting the anti-complement activity.
Keywordred alga Grateloupia livida (Harv.) Yamada mass spectrometry structure
DOI10.1007/s00343-019-8125-x
Indexed BySCI
Language英语
Funding ProjectOcean Public Welfare Scientific Research Project[201405040] ; National Science and Technology Support Project[2013BAB01B01] ; Guangdong Province[2012B090400028] ; Science and Technology Program of Applied Basic Research Projects of Qingdao[15-9-1-74-jch] ; Chinese Academy of Sciences[2012B090400028]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000469401600004
PublisherSCIENCE PRESS
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161635
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan Delin; Zhang Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Zhejiang Univ, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China
3.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
5.State Key Lab Seaweed Bioact Subst, Qingdao 266000, Shandong, Peoples R China
6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang Wenjing,Jin Weihua,Duan Delin,et al. Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(3):806-814.
APA Zhang Wenjing,Jin Weihua,Duan Delin,&Zhang Quanbin.(2019).Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(3),806-814.
MLA Zhang Wenjing,et al."Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.3(2019):806-814.
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