Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers | |
| Yao Jiating1,2,3; Shuai Li4; Li Shengyao5; Xu Caolu5; Wang Xiuliang1,2,3 | |
| 2019-05-01 | |
| 发表期刊 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| 卷号 | 37期号:3页码:783-789 |
| 通讯作者 | Wang Xiuliang(xlwang@qdio.ac.cn) |
| 摘要 | Since the 1980s, Sargassum fusiforme has been cultivated in Zhejiang, South China, and nowadays it becomes one of the important commercial seaweeds in China. With traditions of eating habits in the East Asian countries, this brown alga is used as food, because it contained functional oligo/polysaccharides and chemical components, and was regarded playing roles in antioxidant activities and regulating immunology. Through over 15 years' selection, breeding and cultivation, we obtained three strains with good traits and testified their characters during the production, which included the cultivars with high yield and other two good characters, either all the selected strains were applied in the Sargassum production. To avoid confusion during the selection and nursery, it was preferred to establish one fingerprint for distinguishing the Sargassum cultivars from different strains. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) methods were adopted to analyze the genetic diversities of the selected S. fusiforme strains. With that, one fingerprint with RAPD markers was constructed, and one sequence characterized amplified region (SCAR) marker to S. fusiforme was obtained. It is indicated that the applied fingerprint could be valid in S. fusiforme genetic and gamplasm justification, and will be positive to molecular marker assistance in its selection and cultivation. |
| 关键词 | Sargassum fusiforme random amplified polymorphic DNA (RAPD) inter-simple sequence repeat (ISSR) sequence characterized amplified region (SCAR) genetic analysis |
| DOI | 10.1007/s00343-019-8140-y |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Shandong Key Sci-Technology Research Project[2018SDKJ0302-2] ; Shandong Key Sci-Technology Research Project[2018SDKJ0502-1] ; CAS-Fujian STS Project[2017T3012] ; Doutou Sci-Tech Project[N2006Y11B] ; Shandong Key Sci-Technology Research Project[2018SDKJ0302-2] ; Shandong Key Sci-Technology Research Project[2018SDKJ0502-1] ; CAS-Fujian STS Project[2017T3012] ; Doutou Sci-Tech Project[N2006Y11B] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| WOS类目 | Limnology ; Oceanography |
| WOS记录号 | WOS:000469401600002 |
| 出版者 | SCIENCE PRESS |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/161620 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang Xiuliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Univ, Sch Environm Sci & Engn, Qingdao 266071, Shandong, Peoples R China 5.Dongtou Yangqicai Res Inst, Wenzhou 325700, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Yao Jiating,Shuai Li,Li Shengyao,et al. Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(3):783-789. |
| APA | Yao Jiating,Shuai Li,Li Shengyao,Xu Caolu,&Wang Xiuliang.(2019).Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(3),783-789. |
| MLA | Yao Jiating,et al."Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.3(2019):783-789. |
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