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Functional characterization of two clip-domain serine proteases in the swimming crab Portunus trituberculatus
Liu, Hourong1,3,4; Liu, Yuan1,2,3; Song, Chengwen1,2,3; Ning, Junhao1,5; Cui, Zhaoxia2,5
2019-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume89Pages:98-107
Corresponding AuthorCui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
AbstractClip domain serine proteases (cSPs), a family of multifunctional proteins, play a crucial role in innate immune system. Here, we report the functional characterization of two clip domain serine proteases (PtcSP1 and PtcSP3) from the swimming crab Portunus trituberculatus. The recombinant N-terminal clip domains and the C-terminal SP-like domains of PtcSP1 and PtcSP3 were expressed in Escherichia colt system, and assayed for various biological functions: protease activity, antimicrobial activity, bacterial clearance and microbial-binding activity. The recombinant SP-like domains of PtcSP1 and PtcSP3 exhibited trypsin-like protease activity, while their recombinant clip domains showed strong antibacterial activity and could bind to bacteria and yeast, suggesting the potential roles of PtcSP1 and PtcSP3 in immune defense and pattern recognition. Unlike PtcSP3, PtcSP1 revealed the opsonic activity as shown by a higher bacterial clearance rate of Vibrio alginolyticus coated with the combination of the recombinant clip domain and SP-like domain of PtcSP1 as compared with V. alginolyticus only. Knockdown of PtcSP1 or PtcSP3 by RNA interference resulted in a significant decrease of total phenoloxidase (PO) activity in crab, suggesting that PtcSP1 and PtcSP3 are involved in the proPO system. In addition, suppression of PtcSP1 or PtcSP3 changed the expression of PtALFs and complement-like components. All these findings suggest that PtcSP1 and PtcSP3 are multifunctional immune molecules and perform different protective functions in crab defense.
KeywordPortunus trituberculatus Clip domain serine protease Antimicrobial activity Bacterial clearance Prophenoloxidase activating system Complement-like factors
DOI10.1016/j.fsi.2018.12.047
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900303] ; National Natural Science Foundation of China[41776159] ; National Natural Science Foundation of China[41206147] ; Scientific and Technological Innovation8 Project of Qingdao National Laboratory for Marine Science and Technology, China[2015ASKJ02] ; Natural Science Foundation of Shandong Province, China[ZR2017QD001]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000469897700013
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161602
Collection实验海洋生物学重点实验室
Corresponding AuthorCui, Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Hourong,Liu, Yuan,Song, Chengwen,et al. Functional characterization of two clip-domain serine proteases in the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:98-107.
APA Liu, Hourong,Liu, Yuan,Song, Chengwen,Ning, Junhao,&Cui, Zhaoxia.(2019).Functional characterization of two clip-domain serine proteases in the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,89,98-107.
MLA Liu, Hourong,et al."Functional characterization of two clip-domain serine proteases in the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 89(2019):98-107.
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