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An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP
Sun, Mingzhe1,2,3; Li, Shihao1,2,4; Yu, Kuijie1,2,4; Xiang, Jianhai1,2; Li, Fuhua1,2,4
2019-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume97Pages:57-63
Corresponding AuthorLi, Fuhua(fhli@qdio.ac.cn)
AbstractThe TRIpartite Motif (TRIM) proteins are known to play key roles in cell differentiation, apoptosis, development, autophagy and innate immunity. In the present study, a TRIM9 homolog (named LvTRIM9) was identified from the transcriptome of the Pacific whiteleg shrimp Litopenaeus vannamei. The deduced amino acid sequence of LvTRIM9 possessed typical features of TRIMs, consisting of a RING domain, two B-boxes, a coiled-coil domain, a FN3 domain, and a SPRY domain. The transcript of LvTRIM9 was detected in most tissues of the shrimp. Its expression level was obviously up-regulated at 3, 12 and 24 h post white spot syndrome virus (WSSV) infection. Knockdown of LvTRIM9 gene expression by double-strand RNA mediated interference could lead to a decrease of virus copy number in WSSV-infected shrimp. Yeast two-hybrid analysis showed that LvTRIM9 could directly interact with beta-transducin repeat-containing protein of shrimp (Lv beta-TrCP), an inhibitor of NF-kappa B pathway. Meanwhile, knockdown of LvTRIM9 could also up-regulate the expression levels of LvRelish and downstream production of antimicrobial peptides in the intestine of shrimp. These data indicated that WSSV might hijack the LvTRIM9 for its propagation through inhibition of NF-kappa B pathway and downstream antimicrobial peptides production via interaction of LvTRIM9 with Lv beta-TrCP in shrimp. The study improved our understanding about the impact of E3 ubiquitin ligases on the innate immune signaling pathway of shrimp and its role during WSSV infection.
KeywordTRIM9 beta-TrCP White spot syndrome virus NB-kappa B pathway Antimicrobial peptides
DOI10.1016/j.dci.2019.03.014
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0900502] ; China Agriculture Research System-48 (CARS-48) ; National Natural Science Foundation of China[41776158] ; National Natural Science Foundation of China[31772880] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000468721600007
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161425
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Mingzhe,Li, Shihao,Yu, Kuijie,et al. An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,97:57-63.
APA Sun, Mingzhe,Li, Shihao,Yu, Kuijie,Xiang, Jianhai,&Li, Fuhua.(2019).An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,97,57-63.
MLA Sun, Mingzhe,et al."An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with beta-TrCP".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 97(2019):57-63.
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