Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation | |
| Li, Kecheng1,2,3,4; Green, Andrew R.1; Dinges, Meredith M.1; Larive, Cynthia K.1 | |
| 2019-05-15 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| Volume | 129Pages:744-749 |
| Corresponding Author | Li, Kecheng(lkc@qdio.ac.cn) ; Larive, Cynthia K.(clarive@ucr.edu) |
| Abstract | Chitin oligosaccharides, composed of homogeneous beta(1 -> 4)-linked N-acetyl-D-glucosamine (GlcNAc) sequences, is a well-known elicitor of plant immune defense, and also occur as structural elements of chitosan and nodulation (Nod) factor. Detailed microstructure characterization is required for understanding the function mode of these bioactive molecules. Herein, experimental conditions for detection and elucidation of the H-1 NMR resonances of amide groups in chitin oligosaccharides are presented. The binary mixture of 70% H2O: 30% DMSO-d(6) was found to be the optimal solvent for amide proton measurements in homogeneous GlcNAc sequences, facilitating differentiation of the local chemical microenvironments of all four amide groups of the chitin tetrasaccharide. Experimental evidence that anomeric end-effect triggers amide proton resonance differentiation at the adjacent residue has potential to provide important insights into the solution structure of chitin and other amino sugars containing GlcNAc sequences. (C) 2019 Elsevier B.V. All rights reserved. |
| Keyword | Chitin Structure characterization Amide proton Binary solvent End-effect |
| DOI | 10.1016/j.ijbiomac.2019.02.062 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2017GHY15119] ; China Scholarship Council[201504910405] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS ID | WOS:000466621200078 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/161419 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Li, Kecheng; Larive, Cynthia K. |
| Affiliation | 1.Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,et al. H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,129:744-749. |
| APA | Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,&Larive, Cynthia K..(2019).H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,129,744-749. |
| MLA | Li, Kecheng,et al."H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 129(2019):744-749. |
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