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H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation
Li, Kecheng1,2,3,4; Green, Andrew R.1; Dinges, Meredith M.1; Larive, Cynthia K.1
2019-05-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume129Pages:744-749
Corresponding AuthorLi, Kecheng(lkc@qdio.ac.cn) ; Larive, Cynthia K.(clarive@ucr.edu)
AbstractChitin oligosaccharides, composed of homogeneous beta(1 -> 4)-linked N-acetyl-D-glucosamine (GlcNAc) sequences, is a well-known elicitor of plant immune defense, and also occur as structural elements of chitosan and nodulation (Nod) factor. Detailed microstructure characterization is required for understanding the function mode of these bioactive molecules. Herein, experimental conditions for detection and elucidation of the H-1 NMR resonances of amide groups in chitin oligosaccharides are presented. The binary mixture of 70% H2O: 30% DMSO-d(6) was found to be the optimal solvent for amide proton measurements in homogeneous GlcNAc sequences, facilitating differentiation of the local chemical microenvironments of all four amide groups of the chitin tetrasaccharide. Experimental evidence that anomeric end-effect triggers amide proton resonance differentiation at the adjacent residue has potential to provide important insights into the solution structure of chitin and other amino sugars containing GlcNAc sequences. (C) 2019 Elsevier B.V. All rights reserved.
KeywordChitin Structure characterization Amide proton Binary solvent End-effect
DOI10.1016/j.ijbiomac.2019.02.062
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2017GHY15119] ; China Scholarship Council[201504910405]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000466621200078
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161419
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Kecheng; Larive, Cynthia K.
Affiliation1.Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,et al. H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,129:744-749.
APA Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,&Larive, Cynthia K..(2019).H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,129,744-749.
MLA Li, Kecheng,et al."H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 129(2019):744-749.
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