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| H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation | |
| Li, Kecheng1,2,3,4; Green, Andrew R.1; Dinges, Meredith M.1; Larive, Cynthia K.1 | |
| 2019-05-15 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| 卷号 | 129页码:744-749 |
| 通讯作者 | Li, Kecheng(lkc@qdio.ac.cn) ; Larive, Cynthia K.(clarive@ucr.edu) |
| 摘要 | Chitin oligosaccharides, composed of homogeneous beta(1 -> 4)-linked N-acetyl-D-glucosamine (GlcNAc) sequences, is a well-known elicitor of plant immune defense, and also occur as structural elements of chitosan and nodulation (Nod) factor. Detailed microstructure characterization is required for understanding the function mode of these bioactive molecules. Herein, experimental conditions for detection and elucidation of the H-1 NMR resonances of amide groups in chitin oligosaccharides are presented. The binary mixture of 70% H2O: 30% DMSO-d(6) was found to be the optimal solvent for amide proton measurements in homogeneous GlcNAc sequences, facilitating differentiation of the local chemical microenvironments of all four amide groups of the chitin tetrasaccharide. Experimental evidence that anomeric end-effect triggers amide proton resonance differentiation at the adjacent residue has potential to provide important insights into the solution structure of chitin and other amino sugars containing GlcNAc sequences. (C) 2019 Elsevier B.V. All rights reserved. |
| 关键词 | Chitin Structure characterization Amide proton Binary solvent End-effect |
| DOI | 10.1016/j.ijbiomac.2019.02.062 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2017GHY15119] ; China Scholarship Council[201504910405] ; National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2017GHY15119] ; China Scholarship Council[201504910405] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS记录号 | WOS:000466621200078 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/161419 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Kecheng; Larive, Cynthia K. |
| 作者单位 | 1.Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,et al. H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,129:744-749. |
| APA | Li, Kecheng,Green, Andrew R.,Dinges, Meredith M.,&Larive, Cynthia K..(2019).H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,129,744-749. |
| MLA | Li, Kecheng,et al."H-1 NMR characterization of chitin tetrasaccharide in binary H2O:DMSO solution: Evidence for anomeric end-effect propagation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 129(2019):744-749. |
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| H-1 NMR characteriza（818KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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