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The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles
Wang, M. Q.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, L.1,2,4
2019
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
Volume16Pages:25-33
Corresponding AuthorWang, L.(wanglei@qdio.ac.cn)
AbstractMalectin is a newly discovered lectin of the endoplasmic reticulum (ER) that might be involved in innate immunity. Information about the roles of malectin in innate immunity is scarce. In the present study, a novel malectin gene (designated as CfMal) from the Zhikong scallop Chlamys farreri was identified and characterized. Sequence features, tissue distribution, and temporal expression profiles were investigated to infer the potential functions of CfMal in innate immunity. The complete cDNA sequence of CfMal comprised 1,111 bp and contained an open reading frame of 909 bp, which encoded 302 amino acid residues. A malectin domain and a transmembrane region were identified in the predicted protein sequence. CfMal mRNA transcripts were detectable in hemocytes, muscle, mantle, gill, hepatopancreas, and gonads. CfMal expression was highest in hemocytes. Stimulation with Vibrio splendidus increased CfMal expression in hemocytes, gill, and hepatopancreas. The mRNA transcripts of CfMal and three related genes, including binding immunoglobulin protein, heat shock protein 90 kDa beta member 1 protein and ER degradation enhancing alpha-mannosidase like protein 1, increased in scallop hemocytes during an artificial ER-stress. Our results indicate that CfMal might not only be involved in ER-stress, but may also play a role in innate immunity of scallops.
KeywordChlamys farreri innate immunity malectin
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[U1706209] ; Aoshan Innovation Project in Science and Technology from National Laboratory for Marine Science and Technology[2016ASKJ07] ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; STS Regional Centre Project (Fujian) from the Chinese Academy of Sciences
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000466031500002
PublisherINVERTEBRATE SURVIVAL JOURNAL
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161362
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, L.
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, M. Q.,Wang, B. J.,Liu, M.,et al. The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2019,16:25-33.
APA Wang, M. Q.,Wang, B. J.,Liu, M.,Jiang, K. Y.,&Wang, L..(2019).The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles.ISJ-INVERTEBRATE SURVIVAL JOURNAL,16,25-33.
MLA Wang, M. Q.,et al."The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles".ISJ-INVERTEBRATE SURVIVAL JOURNAL 16(2019):25-33.
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