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Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)
Chen, Xing1,2,3,4; Qiu, Liang1,2,3; Wang, Hailiang1,2,3; Zou, Peizhuo1,2,3; Dong, Xuan1,2,3; Li, Fuhua5; Huang, Jie1,2,3,4
2019-04-01
Source PublicationVIRUSES-BASEL
ISSN1999-4915
Volume11Issue:4Pages:15
Corresponding AuthorHuang, Jie(huangjie@ysfri.ac.cn)
AbstractIn this study, ridgetail white prawnsExopalaemon carinicaudawere infected per os (PO) with debris of Penaeus vannamei infected with shrimp hemocyte iridescent virus (SHIV 20141215), a strain of decapod iridescent virus 1 (DIV1), and via intramuscular injection (IM with raw extracts of SHIV 20141215. The infected E. carinicauda showed obvious clinical symptoms, including weakness, empty gut and stomach, pale hepatopancreas, and partial death with mean cumulative mortalities of 42.5% and 70.8% by nonlinear regression, respectively. Results of TaqMan probe-based real-time quantitative PCR showed that the moribund and surviving individuals with clinical signs of infected E. carinicauda were DIV1-positive. Histological examination showed that there were darkly eosinophilic and cytoplasmic inclusions, of which some were surrounded with or contained tiny basophilic staining, and pyknosis in hemocytes in hepatopancreatic sinus, hematopoietic cells, cuticular epithelium, etc. On the slides of in situ DIG-labeling-loop-mediated DNA amplification (ISDL), positive signals were observed in hematopoietic tissue, stomach, cuticular epithelium, and hepatopancreatic sinus of infected prawns from both PO and IM groups. Transmission electron microscopy (TEM) of ultrathin sections showed that icosahedral DIV1 particles existed in hepatopancreatic sinus and gills of the infected E. carinicauda from the PO group. The viral particles were also observed in hepatopancreatic sinus, gills, pereiopods, muscles, and uropods of the infected E. carinicauda from the IM group. The assembled virions, which mostly distributed along the edge of the cytoplasmic virogenic stromata near cellular membrane of infected cells, were enveloped and approximately 150 nm in diameter. The results of molecular tests, histopathological examination, ISDL, and TEM confirmed that E. carinicauda is a susceptible host of DIV1. This study also indicated that E. carinicauda showed some degree of tolerance to the infection with DIV1 per os challenge mimicking natural pathway.
KeywordSHIV DIV1 Decapodiridovirus Exopalaemon carinicauda susceptibility host ISDL
DOI10.3390/v11040387
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-3] ; Pilot National Laboratory for Marine Science and Technology (Qingdao)[QNLM201706] ; China ASEAN Maritime Cooperation Fund Project ; China Agriculture Research System[CARS-48]
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000467310600084
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161347
Collection实验海洋生物学重点实验室
Corresponding AuthorHuang, Jie
Affiliation1.Chinese Acad Fishery Sci, Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao 266071, Shandong, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Chen, Xing,Qiu, Liang,Wang, Hailiang,et al. Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)[J]. VIRUSES-BASEL,2019,11(4):15.
APA Chen, Xing.,Qiu, Liang.,Wang, Hailiang.,Zou, Peizhuo.,Dong, Xuan.,...&Huang, Jie.(2019).Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1).VIRUSES-BASEL,11(4),15.
MLA Chen, Xing,et al."Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)".VIRUSES-BASEL 11.4(2019):15.
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