Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30 | |
| Wu, Shimei1; Liu, Ge2,3,4; Zhou, Shengnan1; Sha, Zhenxia1; Sun, Chaomin2,3,4 | |
| 2019-04-01 | |
| Source Publication | MARINE DRUGS
 |
| ISSN | 1660-3397 |
| Volume | 17Issue:4Pages:16 |
| Corresponding Author | Sha, Zhenxia(shazhenxia@163.com) ; Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| Abstract | This study was initiated to screen for marine bacterial agents to biocontrol Magnaporthe grisea, a serious fungal pathogen of cereal crops. A bacterial strain, isolated from the cold seep in deep sea, exhibited strong growth inhibition against M. grisea, and the strain was identified and designated as Bacillus sp. CS30. The corresponding antifungal agents were purified by acidic precipitation, sequential methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), and two antifungal peaks were obtained at the final purification step. After analysis by mass spectrometry (MS) and tandem MS, two purified antifungal agents were deduced to belong to the surfactin family, and designated as surfactin CS30-1 and surfactin CS30-2. Further investigation showed that although the antifungal activity of surfactin CS30-1 is higher than that of surfactin CS30-2, both of them induced the increased generation of reactive oxygen species (ROS) and caused serious damage to the cell wall and cytoplasm, thus leading to the cell death of M. grisea. Our results also show the differences of the antifungal activity and antifungal mechanism of the different surfactin homologs surfactin CS30-1 and surfactin CS30-2, and highlight them as potential promising agents to biocontrol plant diseases caused by M. grisea. |
| Keyword | lipopeptide surfactin Bacillus antifungal reactive oxygen species |
| DOI | 10.3390/md17040199 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFC0310800] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051] ; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 100-Talent Project of the Chinese Academy of Sciences |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Chemistry, Medicinal |
| WOS ID | WOS:000467307100006 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/161326 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sha, Zhenxia; Sun, Chaomin |
| Affiliation | 1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wu, Shimei,Liu, Ge,Zhou, Shengnan,et al. Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30[J]. MARINE DRUGS,2019,17(4):16. |
| APA | Wu, Shimei,Liu, Ge,Zhou, Shengnan,Sha, Zhenxia,&Sun, Chaomin.(2019).Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30.MARINE DRUGS,17(4),16. |
| MLA | Wu, Shimei,et al."Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30".MARINE DRUGS 17.4(2019):16. |
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