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Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30
Wu, Shimei1; Liu, Ge2,3,4; Zhou, Shengnan1; Sha, Zhenxia1; Sun, Chaomin2,3,4
2019-04-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:4Pages:16
Corresponding AuthorSha, Zhenxia(shazhenxia@163.com) ; Sun, Chaomin(sunchaomin@qdio.ac.cn)
AbstractThis study was initiated to screen for marine bacterial agents to biocontrol Magnaporthe grisea, a serious fungal pathogen of cereal crops. A bacterial strain, isolated from the cold seep in deep sea, exhibited strong growth inhibition against M. grisea, and the strain was identified and designated as Bacillus sp. CS30. The corresponding antifungal agents were purified by acidic precipitation, sequential methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), and two antifungal peaks were obtained at the final purification step. After analysis by mass spectrometry (MS) and tandem MS, two purified antifungal agents were deduced to belong to the surfactin family, and designated as surfactin CS30-1 and surfactin CS30-2. Further investigation showed that although the antifungal activity of surfactin CS30-1 is higher than that of surfactin CS30-2, both of them induced the increased generation of reactive oxygen species (ROS) and caused serious damage to the cell wall and cytoplasm, thus leading to the cell death of M. grisea. Our results also show the differences of the antifungal activity and antifungal mechanism of the different surfactin homologs surfactin CS30-1 and surfactin CS30-2, and highlight them as potential promising agents to biocontrol plant diseases caused by M. grisea.
Keywordlipopeptide surfactin Bacillus antifungal reactive oxygen species
DOI10.3390/md17040199
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0310800] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051] ; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 100-Talent Project of the Chinese Academy of Sciences
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000467307100006
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161326
Collection实验海洋生物学重点实验室
Corresponding AuthorSha, Zhenxia; Sun, Chaomin
Affiliation1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Shimei,Liu, Ge,Zhou, Shengnan,et al. Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30[J]. MARINE DRUGS,2019,17(4):16.
APA Wu, Shimei,Liu, Ge,Zhou, Shengnan,Sha, Zhenxia,&Sun, Chaomin.(2019).Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30.MARINE DRUGS,17(4),16.
MLA Wu, Shimei,et al."Characterization of Antifungal Lipopeptide Biosurfactants Produced by Marine Bacterium Bacillus sp. CS30".MARINE DRUGS 17.4(2019):16.
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