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Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea
Deng, Yunyan1,2,3; Hu, Zhangxi1,2,3; Chai, Zhaoyang1; Tang, Ying Zhong1,2,3
2019-05-01
Source PublicationJOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
Volume66Issue:3Pages:393-403
Corresponding AuthorTang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
AbstractCSPs, cold shock domain (CSD) containing proteins, are demonstrated to be involved in low temperature responses and various cellular processes under normal growth conditions. Here, we used the cosmopolitan, toxic, and resting cyst-producing dinoflagellate Scrippsiella trochoidea as a representative harmful algal bloom-forming dinoflagellate to investigate the expression patterns of CSP in vegetative cells in response to temperature shocks and in resting cysts, with an objective to probe the possible function of CSP in dinoflagellates. The full-length cDNA of a CSP gene from S. trochoidea (StCSP) was obtained which has a solely N-terminal CSD with conserved nucleic acids binding motifs. The qPCR results together indicated StCSP expression was not modulated by temperature at the transcriptional level and implied this gene may not be associated with temperature stress responses in S. trochoidea as the gene's name implies. However, we observed significantly higher StCSP transcripts in resting cysts (newly formed and maintained in dormancy for different periods of time) than that observed in vegetative cells (at exponential and stationary stages), indicating StCSP is actively expressed during dormancy of S. trochoidea. Taking together our recent transcriptomic work on S. trochoidea into consideration, we postulate that StCSP may play roles during encystment and cyst dormancy of the species.
KeywordHarmful algal blooms resting cyst temperature shock
DOI10.1111/jeu.12681
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000465604100003
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161265
Collection海洋生态与环境科学重点实验室
Corresponding AuthorTang, Ying Zhong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,et al. Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2019,66(3):393-403.
APA Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,&Tang, Ying Zhong.(2019).Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea.JOURNAL OF EUKARYOTIC MICROBIOLOGY,66(3),393-403.
MLA Deng, Yunyan,et al."Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea".JOURNAL OF EUKARYOTIC MICROBIOLOGY 66.3(2019):393-403.
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