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Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea | |
Deng, Yunyan1,2,3; Hu, Zhangxi1,2,3; Chai, Zhaoyang1; Tang, Ying Zhong1,2,3 | |
2019-05-01 | |
发表期刊 | JOURNAL OF EUKARYOTIC MICROBIOLOGY |
ISSN | 1066-5234 |
卷号 | 66期号:3页码:393-403 |
通讯作者 | Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
摘要 | CSPs, cold shock domain (CSD) containing proteins, are demonstrated to be involved in low temperature responses and various cellular processes under normal growth conditions. Here, we used the cosmopolitan, toxic, and resting cyst-producing dinoflagellate Scrippsiella trochoidea as a representative harmful algal bloom-forming dinoflagellate to investigate the expression patterns of CSP in vegetative cells in response to temperature shocks and in resting cysts, with an objective to probe the possible function of CSP in dinoflagellates. The full-length cDNA of a CSP gene from S. trochoidea (StCSP) was obtained which has a solely N-terminal CSD with conserved nucleic acids binding motifs. The qPCR results together indicated StCSP expression was not modulated by temperature at the transcriptional level and implied this gene may not be associated with temperature stress responses in S. trochoidea as the gene's name implies. However, we observed significantly higher StCSP transcripts in resting cysts (newly formed and maintained in dormancy for different periods of time) than that observed in vegetative cells (at exponential and stationary stages), indicating StCSP is actively expressed during dormancy of S. trochoidea. Taking together our recent transcriptomic work on S. trochoidea into consideration, we postulate that StCSP may play roles during encystment and cyst dormancy of the species. |
关键词 | Harmful algal blooms resting cyst temperature shock |
DOI | 10.1111/jeu.12681 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
WOS记录号 | WOS:000465604100003 |
出版者 | WILEY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/161265 |
专题 | 海洋生态与环境科学重点实验室 |
通讯作者 | Tang, Ying Zhong |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
第一作者单位 | 海洋生态与环境科学重点实验室 |
通讯作者单位 | 海洋生态与环境科学重点实验室 |
推荐引用方式 GB/T 7714 | Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,et al. Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2019,66(3):393-403. |
APA | Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,&Tang, Ying Zhong.(2019).Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea.JOURNAL OF EUKARYOTIC MICROBIOLOGY,66(3),393-403. |
MLA | Deng, Yunyan,et al."Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea".JOURNAL OF EUKARYOTIC MICROBIOLOGY 66.3(2019):393-403. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Cloning and Partial (658KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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