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Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea
Deng, Yunyan1,2,3; Hu, Zhangxi1,2,3; Chai, Zhaoyang1; Tang, Ying Zhong1,2,3
2019-05-01
发表期刊JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
卷号66期号:3页码:393-403
通讯作者Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
摘要CSPs, cold shock domain (CSD) containing proteins, are demonstrated to be involved in low temperature responses and various cellular processes under normal growth conditions. Here, we used the cosmopolitan, toxic, and resting cyst-producing dinoflagellate Scrippsiella trochoidea as a representative harmful algal bloom-forming dinoflagellate to investigate the expression patterns of CSP in vegetative cells in response to temperature shocks and in resting cysts, with an objective to probe the possible function of CSP in dinoflagellates. The full-length cDNA of a CSP gene from S. trochoidea (StCSP) was obtained which has a solely N-terminal CSD with conserved nucleic acids binding motifs. The qPCR results together indicated StCSP expression was not modulated by temperature at the transcriptional level and implied this gene may not be associated with temperature stress responses in S. trochoidea as the gene's name implies. However, we observed significantly higher StCSP transcripts in resting cysts (newly formed and maintained in dormancy for different periods of time) than that observed in vegetative cells (at exponential and stationary stages), indicating StCSP is actively expressed during dormancy of S. trochoidea. Taking together our recent transcriptomic work on S. trochoidea into consideration, we postulate that StCSP may play roles during encystment and cyst dormancy of the species.
关键词Harmful algal blooms resting cyst temperature shock
DOI10.1111/jeu.12681
收录类别SCI
语种英语
资助项目National Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404]
WOS研究方向Microbiology
WOS类目Microbiology
WOS记录号WOS:000465604100003
出版者WILEY
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/161265
专题海洋生态与环境科学重点实验室
通讯作者Tang, Ying Zhong
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位海洋生态与环境科学重点实验室
通讯作者单位海洋生态与环境科学重点实验室
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Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,et al. Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2019,66(3):393-403.
APA Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,&Tang, Ying Zhong.(2019).Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea.JOURNAL OF EUKARYOTIC MICROBIOLOGY,66(3),393-403.
MLA Deng, Yunyan,et al."Cloning and Partial Characterization of a Cold Shock Domain-Containing Protein Gene from the Dinoflagellate Scrippsiella trochoidea".JOURNAL OF EUKARYOTIC MICROBIOLOGY 66.3(2019):393-403.
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