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pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection
Guan, Xiao-lu1,2,3; Zhang, Bao-cun4,5; Sun, Li1,2
2019-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume87Pages:220-225
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractMicroRNAs (miRNAs) are a type of small non-coding RNAs that participate in diverse cellular processes including microbial invasion and immune defense. In a previous study, we identified a large amount of Japanese flounder (Paralichthys olivaceus) miRNAs responsive to megalocytivirus infection. In the present study, we examined the function of one of these miRNAs, pol-miR-194a, in association with the infectivity of Edwardsiella tarda, an intracellular bacterial pathogen to many fish species including flounder. We found that pol-miR-194a was induced in expression to a significant extent in the spleen, liver, and gill of Japanese flounder infected by E. tarda. Transfection of flounder cells with pol-miR-194a mimic significantly enhanced the intracellular replication of E. tarda. pol-miR-194a was able to interact specifically with the 3'UTR of IRF7 in a negative manner, resulting in inhibition of IRF7 expression. Consistently, pol-miR-194a significantly blocked the promoter activity of type I interferon. Taken together, these results indicate that pol-miR-194a plays an important role in the regulation of flounder immune response as well as microbial infection, and that pol-miR-194a probably serves as a target for E. tarda to manipulate and escape host immune defense.
KeywordmiRNA Paralichthys olivaceus Edwardsiella tarda Type I interferon Immune escape
DOI10.1016/j.fsi.2019.01.017
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; National Key R&D Program of China[2018YFD0900504] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000464086600025
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161114
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, CAS Ctr Ocean Mega Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Aarhus Univ, Dept Biomed, Aarhus, Denmark
5.Aarhus Univ, Aarhus Res Ctr Innate Immun, Aarhus, Denmark
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Guan, Xiao-lu,Zhang, Bao-cun,Sun, Li. pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:220-225.
APA Guan, Xiao-lu,Zhang, Bao-cun,&Sun, Li.(2019).pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection.FISH & SHELLFISH IMMUNOLOGY,87,220-225.
MLA Guan, Xiao-lu,et al."pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection".FISH & SHELLFISH IMMUNOLOGY 87(2019):220-225.
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