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A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas
Wang, Xiudan1; Zhao, Xiaoli1; Yan, Chunyu1; Jia, Zhihao2,3; Lv, Zhao2,4; Ma, Cuiping1; Wang, Mengqiang2,5,6,7
2019-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume88Pages:318-327
Corresponding AuthorWang, Mengqiang(wangmengqiang@qdio.ac.cn)
AbstractA variety of combinations of leucine-rich repeat (LRR) and immunoglobulin-like (Ig) domains have been found and discovered in invertebrates and vertebrates, but the functions remain largely unexplored. In the present study, a novel LRR and Ig domain-containing protein (LRRIG), CgLRRIG-3, was identified and characterized from oyster Crassostrea gigas. It contained two typical LRR motifs, a LRRNT motif and an Ig domain and PSI-BALST and phylogeny analysis revealed that the sequence of CgLRRIG-3 was most related with leucine-rich repeat neuronal 1 proteins from vertebrate. Its mRNA transcripts were constitutively expressed in muscle, gill, hepatopancreas, mantle, gonad and hemocytes with the highest level in hepatopancreas. The mRNA expression level of CgLRRIG-3 in hemocytes could respond to the stimulations of variety PAMPs including lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C). The recombinant proteins exhibited a wide PAMP binding repertoire to four typical PAMPs and could significantly induce the expression of CgTNF-1 and CgIL17-5 as well as increase phagocytosis in primary cultured oyster hemocytes. In hepatopancreas, CgLRRIG-3 was mainly distributed in the basolateral membrane of digestive tubule and the hemocoel sinusoid between the digestive tubules. And in hemocytes, the positive signal was mainly distributed in a special group of granulocytes. These results collectively indicated that CgLRRIG-3 could not only function as an immune effector.
KeywordCrassostrea gigas Leucine-rich repeat domain Immunoglobulin domain LRRIG Pattern recognition receptor Immune effector
DOI10.1016/j.fsi.2019.03.003
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31800414] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2018N01] ; Research Initial Funding Project for Doctors in Qingdao University of Science and Technology[010022908]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000465060500038
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161105
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Mengqiang
Affiliation1.Qingdao Univ Sci & Technol, Shandong Prov Key Lab Biochem Engn, Coll Marine Sci & Biol Engn, Qingdao 266042, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
3.Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China
6.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
7.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiudan,Zhao, Xiaoli,Yan, Chunyu,et al. A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:318-327.
APA Wang, Xiudan.,Zhao, Xiaoli.,Yan, Chunyu.,Jia, Zhihao.,Lv, Zhao.,...&Wang, Mengqiang.(2019).A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,88,318-327.
MLA Wang, Xiudan,et al."A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 88(2019):318-327.
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