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Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research
Li, Qianqian1; Zhang, Litao1,2; Pang, Tong1,2; Liu, Jianguo1,2
2019-04-03
Source PublicationEUROPEAN JOURNAL OF PHYCOLOGY
ISSN0967-0262
Volume54Issue:2Pages:162-174
Corresponding AuthorLiu, Jianguo(jgliu@qdio.ac.cn)
AbstractKappaphycus alvarezii provides an important raw material for the extraction of kappa-carrageenan. Comparative transcriptome analysis of K. alvarezii after 24 h extreme temperature treatments (17 degrees C and 34 degrees C) was performed to uncover the molecular mechanisms controlling temperature-related photosynthesis regulation. Optimum temperature (28 degrees C) was employed as the control. After de novo assembly of these three transcriptome samples and searching the assembled transcripts by BLAST against the NCBI nr databases, 11,537 top-hit transcripts were assigned as unigenes. Through comparisons, 302 significantly differentially expressed genes were identified. Twenty-three of the differentially expressed unigenes encoded photosystem antenna protein subunits and a further 25 were associated more broadly with photosynthesis functions. Compared to the light-harvesting complex (LHC) protein, the expression of phycobiliproteins (PBPs) was stable after extreme low and high temperature (ELT/EHT) treatments. Both ELT and EHT were found to inhibit the expression of subunits of photosystem II (PsbA, PsbC, Psb27, PsbW, PsbS), photosystem I (PsaF, PsaG, PsaK) and photosynthetic electron transport (PetE). These results were confirmed through targeted analysis of the mRNA and protein abundance of individual proteins, and were congruent with the results of chlorophyll a fluorescence (OJIP) measurements performed with temperature-stressed lines. In conclusion, similarities and differences in the behaviours of photosynthesis-related genes were summarized to provide opportunities for understanding the responses of K. alvarezii after extreme temperature treatments.
KeywordAntenna protein extreme temperature Kappaphycus alvarezii photosynthesis thylakoid membrane proteins transcriptome
DOI10.1080/09670262.2018.1536283
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702366] ; National Natural Science Foundation of China[41306154] ; Open Foundation of Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences[LMB17011007]
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000463785700003
PublisherTAYLOR & FRANCIS LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161093
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Jianguo
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Qianqian,Zhang, Litao,Pang, Tong,et al. Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2019,54(2):162-174.
APA Li, Qianqian,Zhang, Litao,Pang, Tong,&Liu, Jianguo.(2019).Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research.EUROPEAN JOURNAL OF PHYCOLOGY,54(2),162-174.
MLA Li, Qianqian,et al."Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research".EUROPEAN JOURNAL OF PHYCOLOGY 54.2(2019):162-174.
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