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A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles
Wang, Yan1,3; Wang, Mengqiang1,4; Wang, Baojie1; Liu, Mei1; Jiang, Keyong1; Hou, Xuguang5; Wang, Lei1,2,6
2019-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume88Pages:135-141
Corresponding AuthorWang, Lei(wanglei@qdio.ac.cn)
AbstractTetraspanins belong to the transmembrane 4 superfamily (TM4SF), and play crucial roles in immune responses. In the present study, a novel tetraspanin gene (designated MmTSPAN) was cloned and characterized from the hard clam Meretrix meretrix. The complete cDNA sequence of MmTSPAN contained an open reading frame (ORF) of 816 bp, which encoded a protein of 271 amino acids. MmTSPAN exhibited highly similarity with previously identified tetraspanins from other species. It contained four transmembrane domains (12-35 aa, 69-92 aa, 99-123 aa and 238-261 aa), characteristic CCG motif and four conservative cysteine residues. The mRNA transcripts of MmTSPAN were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. Temporal transcriptional levels in the hepatopancreas revealed significant up-regulation of MmTSPAN by Vibrio splendidus stimulation, with a 3.14-fold increase at 6h compared to the control, and reaching 32.98-fold at 24 h. These results provide useful information for further study of the function of tetraspanin in the innate immune system of M. meretrix, and may offer a new therapeutic target for diseases of M. meretrix.
KeywordMeretrix meretrix Tetraspanin Innate immunity
DOI10.1016/j.fsi.2019.01.048
Indexed BySCI
Language英语
Funding ProjectMajor Special Science and Technology Project of Shandong Province, China[2018SDKJ0] ; Science and Technology Development Fund Project of Shinan district of Qingdao city, China[2018-4-001-ZH]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000465060500016
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161078
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Shandong Univ Weihai, Marine Coll, Weihai 264209, Peoples R China
6.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Yan,Wang, Mengqiang,Wang, Baojie,et al. A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:135-141.
APA Wang, Yan.,Wang, Mengqiang.,Wang, Baojie.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles.FISH & SHELLFISH IMMUNOLOGY,88,135-141.
MLA Wang, Yan,et al."A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles".FISH & SHELLFISH IMMUNOLOGY 88(2019):135-141.
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