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Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut
Du, Yang1,2; Wang, Baojie1,2; Jiang, Keyong1,2; Wang, Mengqiang1,2; Zhou, Shuhong2,3; Liu, Mei1,2; Wang, Lei1,2,4
2019-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume87Pages:853-870
Corresponding AuthorLiu, Mei(liumei@qdio.ac.cn) ; Wang, Lei(wanglei@qdio.ac.cn)
AbstractIn order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCI) treatment The results showed that the shrimp fed with normal HC-2 produced much higher immune and digestion related enzymes than the control group or LiCl-treated HC-2 group to defense the Vibrlo parahaemolyticus El infection. We obtained total over 275,099 unigenes from L. vannamei midgut, 981 genes were significant differentially expressed in normal HC-2 group compared with control, 1314 genes were significant differentially expressed in LiCI-treated HC-2 group compared with control, and 1689 genes were significant differentially expressed in LiCl-treated HC-2 group compared with normal HC-2 group. The GO/KEGG enrichment analysis of the significantly different genes demonstrated that L. vannamei fed with normal HC-2 induced immune-related, signal transduction, ion homeostasis, cell-cell adhesion, response stress/stimulus, vascular endothelial growth factor and peritrophin genes up-regulation, which were important genes involved in improving the shrimp intestine immune response, nutrition and growth performance, and bacteria adhesion and colonization, but these genes were suppressed in the midgut of shrimp fed with deprived surface proteins bacteria. Taken together, these results indicated that the surface proteins were essential for HC-2 executing probiotic effects in midgut of shrimp. Our data contribute to improve the current understanding of host Lactobacillus interaction and the probiotic mechanisms in shrimps.
KeywordLitopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Transcriptome
DOI10.1016/j.fsi.2019.02.027
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31802309] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] ; China Postdoctoral Science Foundation[2018M632736]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000464086600094
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161069
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Mei; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Yang,Wang, Baojie,Jiang, Keyong,et al. Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:853-870.
APA Du, Yang.,Wang, Baojie.,Jiang, Keyong.,Wang, Mengqiang.,Zhou, Shuhong.,...&Wang, Lei.(2019).Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut.FISH & SHELLFISH IMMUNOLOGY,87,853-870.
MLA Du, Yang,et al."Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut".FISH & SHELLFISH IMMUNOLOGY 87(2019):853-870.
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