Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei

doi:10.1016/j.fsi.2018.10.019

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei
	Wang, Zhiwei1 ; Li, Shihao 1,2,3; Yu, Yang1,2,3 ; Yu, Kuijie 1,2,3; Zhang, Xiaojun 1,2,3; Xiang, Jianhai1,2,3 ; Li, Fuhua 1,2,3
	2019
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	84 页码:259-268
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要	Vascular endothelial growth factor (VEGF) signaling pathway induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Although three VEGF and two VEGF receptor genes have been identified in Litopenaeus vannamei and demonstrated their roles in WSSV infection, another two novel VEGF genes (LvVEGF4, LvVEGF5) were isolated and their involvements in the WSSV infection of shrimp were studied in the present study. The deduced amino acid sequences of both LvVEGF4 and LvVEGF5 contained a signal peptide, a typical PDGF/VEGF domain and a cysteine knot motif (CXCXCX). Tissue distribution analysis showed that LvVEGF4 was predominantly expressed in gill and hemocytes, while LvVEGF5 was mainly detected in hemocytes and intestine. WSSV infection could cause up-regulation of the transcriptional levels of LvVEGF4 and LvVEGF5. Their functions were studied by double-strand RNA interference. The results showed that knock-down of LvVEGF4 and LvVEGF5 led to a decrease of the viral copy number in WSSV infected shrimp. Yeast two-hybrid analysis showed that both LvVEGF4 and LvVEGF5 could interact with LvVEGFR1 rather than LvVEGFR2. In addition, knock-down of LvVEGF4 and LvVEGF5 could reduce the expressional levels of downstream genes FAK and P13K. The present study provides new clues in demonstrating that the VEGF signaling pathway is involved in the process of WSSV infection in shrimp.
关键词	VEGF signaling pathway Shrimp White spot syndrome virus RNA interference Protein interaction
DOI	10.1016/j.fsi.2018.10.019
收录类别	SCI
语种	英语
资助项目	General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; General Program of National Natural Science Foundation of China[31502161] ; China Agriculture Research System-48[CARS-48] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; General Program of National Natural Science Foundation of China[31502161] ; China Agriculture Research system-48[CARS-48] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000457666800030
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160886
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Zhiwei,Li, Shihao,Yu, Yang,et al. Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:259-268.
APA	Wang, Zhiwei.,Li, Shihao.,Yu, Yang.,Yu, Kuijie.,Zhang, Xiaojun.,...&Li, Fuhua.(2019).Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,84,259-268.
MLA	Wang, Zhiwei,et al."Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 84(2019):259-268.

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