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Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei
Wang, Zhiwei1; Li, Shihao1,2,3; Yu, Yang1,2,3; Yu, Kuijie1,2,3; Zhang, Xiaojun1,2,3; Xiang, Jianhai1,2,3; Li, Fuhua1,2,3
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume84Pages:259-268
Corresponding AuthorLi, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
AbstractVascular endothelial growth factor (VEGF) signaling pathway induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Although three VEGF and two VEGF receptor genes have been identified in Litopenaeus vannamei and demonstrated their roles in WSSV infection, another two novel VEGF genes (LvVEGF4, LvVEGF5) were isolated and their involvements in the WSSV infection of shrimp were studied in the present study. The deduced amino acid sequences of both LvVEGF4 and LvVEGF5 contained a signal peptide, a typical PDGF/VEGF domain and a cysteine knot motif (CXCXCX). Tissue distribution analysis showed that LvVEGF4 was predominantly expressed in gill and hemocytes, while LvVEGF5 was mainly detected in hemocytes and intestine. WSSV infection could cause up-regulation of the transcriptional levels of LvVEGF4 and LvVEGF5. Their functions were studied by double-strand RNA interference. The results showed that knock-down of LvVEGF4 and LvVEGF5 led to a decrease of the viral copy number in WSSV infected shrimp. Yeast two-hybrid analysis showed that both LvVEGF4 and LvVEGF5 could interact with LvVEGFR1 rather than LvVEGFR2. In addition, knock-down of LvVEGF4 and LvVEGF5 could reduce the expressional levels of downstream genes FAK and P13K. The present study provides new clues in demonstrating that the VEGF signaling pathway is involved in the process of WSSV infection in shrimp.
KeywordVEGF signaling pathway Shrimp White spot syndrome virus RNA interference Protein interaction
DOI10.1016/j.fsi.2018.10.019
Indexed BySCI
Language英语
Funding ProjectGeneral Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; General Program of National Natural Science Foundation of China[31502161] ; China Agriculture Research system-48[CARS-48] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000457666800030
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160886
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Zhiwei,Li, Shihao,Yu, Yang,et al. Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:259-268.
APA Wang, Zhiwei.,Li, Shihao.,Yu, Yang.,Yu, Kuijie.,Zhang, Xiaojun.,...&Li, Fuhua.(2019).Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,84,259-268.
MLA Wang, Zhiwei,et al."Identification and characterization of two novel vascular endothelial growth factor genes in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 84(2019):259-268.
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