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Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus
Ning, Junhao1,3,4; Liu, Yuan1,2,3; Gao, Fengtao1,2,3; Liu, Hourong1,3,4; Cui, Zhaoxia1,2,3
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume84Pages:970-978
Corresponding AuthorLiu, Yuan(liuyuan@qdio.ac.cn) ; Cui, Zhaoxia(zhxcui@qdio.ac.cn)
AbstractThe receptor for the globular head of complement component C1q, gC1qR, is a multifunctional and multiligand binding protein with a crucial role in host defense. In the present study, a full-length cDNA sequence of a gC1qR homolog (PtgC1qR) in Portunus trituberculoms was identified. PtgC1qR was a 268-amino-acid polypeptide with a conserved MAM33 domain and a mitochondrial targeting sequence in the first 56 amino acids. The transcripts of PtgC1qR were detected in all examined tissues with the highest level detected in the hepatopancreas. Compared with other early embryonic stages, PtgC1qR was highly expressed in the fertilized eggs and embryos at the cleavage stage, which suggest PtgC1qR may be a maternal gene. The transcripts of PtgC1qR in hemocytes exhibited time-dependent response expression pattern after challenged with bacteria (Vibrio alginolyticus, Micrococcus luteus) and fungi (Pichia pastoris). Moreover, the recombinant PtgC1qR (rPtgC1qR) exhibited strong antibacterial activity and microbial-binding activity, suggesting its crucial role in immune defense and recognition. Further phenoloxidase (PO) assay showed that rPtgC1qR could suppress the crab PO activity in vitro in a dose-dependent manner, and it could result in nearly 100% inhibition of PO activity under the concentration of 11.65 mu M. Knockdown of PtgC1qR could significantly enhance the expression of serine protease related genes (PtSP1-3 and PISPH), proPO-associated genes (PiproPO and PtPPAF) and C3-like genes (Pta2M1 and PtTEP). However, the phagocytosis related genes (PtMyosin, PtRab5 and PtArp) and Pta2M2 were significantly down-regulated in the PtgC1qR silenced crabs. These findings together demonstrate that PtgC1qR might function in crab immune response via its antibacterial activity, immune recognition or regulating the proPO system, complement pathway and phagocytosis.
KeywordPortunus trituberculatus gC1qR Expression profiles Antimicrobial activity Microbial binding activity Prophenoloxidase activating system
DOI10.1016/j.fsi.2018.11.005
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41776159] ; National Natural Science Foundation of China[41206147] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000457666800105
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160868
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Yuan; Cui, Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ning, Junhao,Liu, Yuan,Gao, Fengtao,et al. Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:970-978.
APA Ning, Junhao,Liu, Yuan,Gao, Fengtao,Liu, Hourong,&Cui, Zhaoxia.(2019).Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,84,970-978.
MLA Ning, Junhao,et al."Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 84(2019):970-978.
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