Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus | |
| Ning, Junhao1,3,4; Liu, Yuan1,2,3; Gao, Fengtao1,2,3; Liu, Hourong1,3,4; Cui, Zhaoxia1,2,3 | |
| 2019 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 84Pages:970-978 |
| Corresponding Author | Liu, Yuan(liuyuan@qdio.ac.cn) ; Cui, Zhaoxia(zhxcui@qdio.ac.cn) |
| Abstract | The receptor for the globular head of complement component C1q, gC1qR, is a multifunctional and multiligand binding protein with a crucial role in host defense. In the present study, a full-length cDNA sequence of a gC1qR homolog (PtgC1qR) in Portunus trituberculoms was identified. PtgC1qR was a 268-amino-acid polypeptide with a conserved MAM33 domain and a mitochondrial targeting sequence in the first 56 amino acids. The transcripts of PtgC1qR were detected in all examined tissues with the highest level detected in the hepatopancreas. Compared with other early embryonic stages, PtgC1qR was highly expressed in the fertilized eggs and embryos at the cleavage stage, which suggest PtgC1qR may be a maternal gene. The transcripts of PtgC1qR in hemocytes exhibited time-dependent response expression pattern after challenged with bacteria (Vibrio alginolyticus, Micrococcus luteus) and fungi (Pichia pastoris). Moreover, the recombinant PtgC1qR (rPtgC1qR) exhibited strong antibacterial activity and microbial-binding activity, suggesting its crucial role in immune defense and recognition. Further phenoloxidase (PO) assay showed that rPtgC1qR could suppress the crab PO activity in vitro in a dose-dependent manner, and it could result in nearly 100% inhibition of PO activity under the concentration of 11.65 mu M. Knockdown of PtgC1qR could significantly enhance the expression of serine protease related genes (PtSP1-3 and PISPH), proPO-associated genes (PiproPO and PtPPAF) and C3-like genes (Pta2M1 and PtTEP). However, the phagocytosis related genes (PtMyosin, PtRab5 and PtArp) and Pta2M2 were significantly down-regulated in the PtgC1qR silenced crabs. These findings together demonstrate that PtgC1qR might function in crab immune response via its antibacterial activity, immune recognition or regulating the proPO system, complement pathway and phagocytosis. |
| Keyword | Portunus trituberculatus gC1qR Expression profiles Antimicrobial activity Microbial binding activity Prophenoloxidase activating system |
| DOI | 10.1016/j.fsi.2018.11.005 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41776159] ; National Natural Science Foundation of China[41206147] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000457666800105 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160868 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Yuan; Cui, Zhaoxia |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Ning, Junhao,Liu, Yuan,Gao, Fengtao,et al. Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:970-978. |
| APA | Ning, Junhao,Liu, Yuan,Gao, Fengtao,Liu, Hourong,&Cui, Zhaoxia.(2019).Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,84,970-978. |
| MLA | Ning, Junhao,et al."Characterization and functional analysis of a novel gC1qR in the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 84(2019):970-978. |
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