Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation

doi:10.3390/md17010050

IOCAS-IR > 实验海洋生物学重点实验室

	Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation
	Wang, Ju 1,2,3; Liu, Ge 1,2,4; Ma, Weiping 5; Lu, Zhongxia 5; Sun, Chaomin 1,2,4
	2019
Source Publication	MARINE DRUGS
ISSN	1660-3397
Volume	17 Issue:1 Pages:15
Corresponding Author	Sun, Chaomin(sunchaomin@qdio.ac.cn)
Abstract	Our previous results suggested that EPS11, a novel marine bacterial polysaccharide, might be a potential drug candidate for human non-small cell lung carcinoma treatment. In this study, we further investigate the anticancer mechanisms against liver cancer and the anti-metastatic effects in vivo of EPS11. Firstly, we found that EPS11 exerts cytotoxic effects via blocking cell adhesion and destroying filiform structure formation in Huh7.5 cells. Moreover, mass spectrometry-based proteomic analysis of EPS11-treated Huh7.5 cells revealed that expression of many adhesion-related proteins was significantly changed. It is noteworthy that the expression of CD99, a key factor related to cell adhesion, migration and cell death, is remarkably down-regulated after EPS11 treatment. Importantly, over-expression of CD99 partly rescues cell death rate, and improves cell adhesion and migration ability in Huh7.5 treated by EPS11. Thus, we propose that CD99 is a potential action target of EPS11, inhibiting cancer cell proliferation, adhesion and migration. Notably, administration of EPS11 simultaneously with tumor induction evidently reduces tumor nodule formation in the lungs, which strongly indicates that EPS11 has anti-metastatic effects in vivo. Taken together, our results suggest that EPS11 inhibits liver cancer cell growth via blocking cell adhesion and attenuating filiform structure formation, and has potential as an anti-cancer drug, targeting metastasis of cancer cells, in the future.
Keyword	polysaccharide EPS11 cancer filiform structure adhesion CD99 metastasis
DOI	10.3390/md17010050
Indexed By	SCI
Language	英语
Funding Project	National Key R&D Program of China[2018YFC0310800] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; Tai Shan Young Scholar Foundation of Shandong Province[tsqn20161051] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; 100-Talent Project of the Chinese Academy of Sciences
WOS Research Area	Pharmacology & Pharmacy
WOS Subject	Chemistry, Medicinal
WOS ID	WOS:000458053200050
Publisher	MDPI
Citation statistics
Document Type	期刊论文
Identifier	http://ir.qdio.ac.cn/handle/337002/160859
Collection	实验海洋生物学重点实验室
Corresponding Author	Sun, Chaomin
Affiliation	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China
First Author Affilication	Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author Affilication	Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation GB/T 7714	Wang, Ju,Liu, Ge,Ma, Weiping,et al. Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation[J]. MARINE DRUGS,2019,17(1):15.
APA	Wang, Ju,Liu, Ge,Ma, Weiping,Lu, Zhongxia,&Sun, Chaomin.(2019).Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation.MARINE DRUGS,17(1),15.
MLA	Wang, Ju,et al."Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth and Metastasis via Blocking Cell Adhesion and Attenuating Filiform Structure Formation".MARINE DRUGS 17.1(2019):15.

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