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Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method
Li, Qiuyu1,2,3,4; Zhao, Weihong1,2,3,4; Miao, Hui1,2,3; Han, Xiaotian1,2,3
2019-02-01
发表期刊MARINE POLLUTION BULLETIN
ISSN0025-326X
卷号139页码:136-140
通讯作者Zhao, Weihong(whzhao@qdio.ac.cn)
摘要This work attempted to prolong the validity of the molybdate mixed solution and ascorbic acid solution used in the phosphomolybdenum blue spectrophotometric method by improving their preservation according to the influence factors. The results showed that the molybdate mixed solution can be directly preserved in darkness with validity over half a year. The ascorbic acid solution is influenced by light, temperature, pH, metal ions, oxygen, and bacteria. The validity of ascorbic acid is shortened as the temperature rises. Through keeping in darkness, adding complexing agents, adjusting pH, removing oxygen and sterilization, the validity of ascorbic acid solution was prolonged to over 2.7 times under 4 degrees C and over 5 times under 25 degrees C. At the same time, the hybrid solution of ascorbic acid solution and molybdate mixed solution should be preserved separately, otherwise the using effect is poor.
关键词Marine phosphate Marine phosphate detection Phosphomolybdenum blue spectrophotometric method Reagents preservation
DOI10.1016/j.marpolbul.2018.12.011
收录类别SCI
语种英语
资助项目Open Financial Grant from Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0410] ; Joint Funds of the National Natural Science Foundation of China[U1606404] ; State Oceanic Administration, People's Republic of China ; Open Financial Grant from Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0410] ; Joint Funds of the National Natural Science Foundation of China[U1606404] ; State Oceanic Administration, People's Republic of China
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目Environmental Sciences ; Marine & Freshwater Biology
WOS记录号WOS:000458227800017
出版者PERGAMON-ELSEVIER SCIENCE LTD
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/160844
专题海洋生态与环境科学重点实验室
通讯作者Zhao, Weihong
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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GB/T 7714
Li, Qiuyu,Zhao, Weihong,Miao, Hui,et al. Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method[J]. MARINE POLLUTION BULLETIN,2019,139:136-140.
APA Li, Qiuyu,Zhao, Weihong,Miao, Hui,&Han, Xiaotian.(2019).Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method.MARINE POLLUTION BULLETIN,139,136-140.
MLA Li, Qiuyu,et al."Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method".MARINE POLLUTION BULLETIN 139(2019):136-140.
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