Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22 | |
| Li, Yi-qun1,2,3,4; Sun, Li1,2,3; Li, Jun2,3,5 | |
| 2019 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 84Pages:138-147 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) ; Li, Jun(jli@lssu.edu) |
| Abstract | B cells in fish are proven to be endocytic and have a great contribution to innate immunity like phagocytosis. In this study, the endocytic capacity and the corresponding internalization pathways of IgM(+) B cells in Japanese flounder (Paralichthys olivaceus) were investigated. The results showed that IgM(+) B cells in peripheral blood leukocytes (PBL) and splenic leukocytes (SL) exhibited different abilities to ingest 0.5 mu m and 1 pm latex beads through macropinocytosis-dependent endocytic pathway. Japanese flounder CD22 (PoCD22) co-stimulatory signals were identified to be essential for the innate immune responses in B cells. Most of IgM+ B cells and some IgM(-) cells were demonstrated to be PoCD22 positive. When PoCD22 was blocked by antibody, the endocytic activities and reactive oxygen species (ROS) activities of SL IgM(+) B cells were significantly increased, while the endocytic and ROS activities of PBL IgM(+) B cells were significant decreased. These results collectively suggest that Japanese flounder IgM(+) B cells are able to employ macropinocytosis-dependent endocytic pathway, which is under the regulation of CD22. |
| Keyword | Paralichthys olivaceus IgM(+) B cells Endocytosis Macropinocytosis CD22 B cell regulation |
| DOI | 10.1016/j.fsi.2018.09.068 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31528019] ; AoShan Talents Program from Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000457666800017 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160826 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li; Li, Jun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Lake Super State Univ, Sch Biol Sci, 650 W Easterday Ave, Sault Ste Marie, MI 49783 USA |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Yi-qun,Sun, Li,Li, Jun. Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:138-147. |
| APA | Li, Yi-qun,Sun, Li,&Li, Jun.(2019).Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22.FISH & SHELLFISH IMMUNOLOGY,84,138-147. |
| MLA | Li, Yi-qun,et al."Macropinocytosis-dependent endocytosis of Japanese flounder IgM(+) B cells and its regulation by CD22".FISH & SHELLFISH IMMUNOLOGY 84(2019):138-147. |
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