Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways | |
Yang, Yue1,2,3,4; Xing, Ronge1,2,4 ; Liu, Song1,2,4; Qin, Yukun1,2,4; Li, Kecheng1,2,4; Yu, Huahua1,2,4; Li, Pengcheng1,2,4
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| 2019 | |
| Source Publication | MARINE DRUGS
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| ISSN | 1660-3397 |
| Volume | 17Issue:1Pages:12 |
| Corresponding Author | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| Abstract | Chitooligosaccharides (COS), the hydrolyzed products of chitin and chitosan, can be obtained by various methods. In this study, water-soluble COS were prepared from - and -chitosan by microwave-assisted degradation and their immunostimulatory effects were investigated in RAW 264.7 macrophages. The results indicated that -COS were more active than -COS in promoting the production of nitric oxide (NO) and cytokines, such as tumor necrosis factor- (TNF-) and interleukin 6 (IL-6). Quantitative real-time reverse transcription polymerase chain reaction and Western blotting indicated that COS also enhanced the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-. Further analyses demonstrated that COS induced the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38, p85 and Akt, and the nuclear translocation of p65, indicating that they are able to activate the mitogen-activated protein kinases (MAPKs) and phosphoinositide 3-kinases (PI3K)/Akt signaling pathways dependent on nuclear factor (NF)-B activation. In conclusion, COS activate RAW 264.7 cells via the MAPK and PI3K/Akt signaling pathways and are potential novel immune potentiators. |
| Keyword | chitooligosaccharide immunostimulatory activity RAW 264 7 cells mitogen-activated protein kinases (MAPK) phosphoinositide 3-kinases (PI3K) Akt |
| DOI | 10.3390/md17010036 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFC0311305] ; Special projects of Foshan science and technology innovation team[2017IT100054] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Key Research and Development Program of Shandong Province[2017YYSP018] |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Chemistry, Medicinal |
| WOS ID | WOS:000458053200036 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160808 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Xing, Ronge; Li, Pengcheng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways[J]. MARINE DRUGS,2019,17(1):12. |
| APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2019).Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways.MARINE DRUGS,17(1),12. |
| MLA | Yang, Yue,et al."Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways".MARINE DRUGS 17.1(2019):12. |
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